Cryo-electron microscopy of adeno-associated virus
Adeno-associated virus (AAV) has a single-stranded DNA genome encapsidated in a small
icosahedrally symmetric protein shell with 60 subunits. AAV is the leading delivery vector in …
icosahedrally symmetric protein shell with 60 subunits. AAV is the leading delivery vector in …
Automated data collection in single particle electron microscopy
YZ Tan, A Cheng, CS Potter… - Journal of Electron …, 2015 - academic.oup.com
Automated data collection is an integral part of modern workflows in single particle electron
microscopy (EM) research. This review surveys the software packages available for …
microscopy (EM) research. This review surveys the software packages available for …
Adeno-associated virus 9 structural rearrangements induced by endosomal trafficking pH and glycan attachment
Adeno-associated viruses (AAVs) are small nonenveloped single-stranded DNA (ssDNA)
viruses that are currently being developed as gene therapy biologics. After cell entry, AAVs …
viruses that are currently being developed as gene therapy biologics. After cell entry, AAVs …
Comparative analysis of the capsid structures of AAVrh. 10, AAVrh. 39, and AAV8
Adeno-associated viruses (AAVs) from clade E are often used as vectors in gene delivery
applications. This clade includes rhesus isolate 10 (AAVrh. 10) and 39 (AAVrh. 39) which …
applications. This clade includes rhesus isolate 10 (AAVrh. 10) and 39 (AAVrh. 39) which …
Structural heterogeneity in pre-40S ribosomes
Late-stage 40S ribosome assembly is a highly regulated dynamic process that occurs in the
cytoplasm, alongside the full translation machinery. Seven assembly factors (AFs) regulate …
cytoplasm, alongside the full translation machinery. Seven assembly factors (AFs) regulate …
The integrative role of cryo electron microscopy in molecular and cellular structural biology
I Orlov, AG Myasnikov, L Andronov… - Biology of the …, 2017 - Wiley Online Library
After gradually moving away from preparation methods prone to artefacts such as plastic
embedding and negative staining for cell sections and single particles, the field of cryo …
embedding and negative staining for cell sections and single particles, the field of cryo …
The 2.8 Å electron microscopy structure of adeno-associated virus-DJ bound by a heparinoid pentasaccharide
Q **e, JM Spear, AJ Noble, DR Sousa… - … Therapy Methods & …, 2017 - cell.com
Atomic structures of adeno-associated virus (AAV)-DJ, alone and in complex with
fondaparinux, have been determined by cryoelectron microscopy at 3 Å resolution. The …
fondaparinux, have been determined by cryoelectron microscopy at 3 Å resolution. The …
Adeno-associated virus (AAV-DJ)—cryo-EM structure at 1.56 Å resolution
Adeno-associated virus is the leading viral vector for gene therapy. AAV-DJ is a recombinant
variant developed for tropism to the liver. The AAV-DJ structure has been determined to 1.56 …
variant developed for tropism to the liver. The AAV-DJ structure has been determined to 1.56 …
Structural insights into human bocaparvoviruses
Bocaparvoviruses are emerging pathogens of the Parvoviridae family. Human bocavirus 1
(HBoV1) causes severe respiratory infections and HBoV2 to HBoV4 cause gastrointestinal …
(HBoV1) causes severe respiratory infections and HBoV2 to HBoV4 cause gastrointestinal …
[PDF][PDF] Throughput and resolution with a next-generation direct electron detector
Direct electron detectors (DEDs) have revolutionized cryo-electron microscopy (cryo-EM) by
facilitating the correction of beam-induced motion and radiation damage, and also by …
facilitating the correction of beam-induced motion and radiation damage, and also by …