Abstract The Human Developmental Cell Atlas (HDCA) initiative, which is part of the Human
Cell Atlas, aims to create a comprehensive reference map of cells during development. This …

Tissue clearing of gross anatomical samples was first described more than a century ago
and has only recently found widespread use in the field of microscopy. This renaissance has …

Neutrophils are increasingly recognized as key players in the tumor immune response and
are associated with poor clinical outcomes. Despite recent advances characterizing the …

Spatial transcriptomic technologies promise to resolve cellular wiring diagrams of tissues in
health and disease, but comprehensive map** of cell types in situ remains a challenge …

A central challenge in biology is obtaining high-content, high-resolution information while
analyzing tissue samples at volumes relevant to disease progression. We address this here …

Imaging intact human organs from the organ to the cellular scale in three dimensions is a
goal of biomedical imaging. To meet this challenge, we developed hierarchical phase …

Whole-body imaging techniques play a vital role in exploring the interplay of physiological
systems in maintaining health and driving disease. We introduce wildDISCO, a new …

Accurate segmentation of tubular, network-like structures, such as vessels, neurons, or
roads, is relevant to many fields of research. For such structures, the topology is their most …

Peripheral sensory neurons widely innervate various tissues to continuously monitor and
respond to environmental stimuli. Whether peripheral sensory neurons innervate the spleen …

Spatial molecular profiling of complex tissues is essential to investigate cellular function in
physiological and pathological states. However, methods for molecular analysis of large …