Transient plant transformation mediated by Agrobacterium tumefaciens: Principles, methods and applications

P Krenek, O Samajova, I Luptovciak… - Biotechnology …, 2015 - Elsevier
Agrobacterium tumefaciens is widely used as a versatile tool for development of stably
transformed model plants and crops. However, the development of Agrobacterium based …

Optical tweezers analysis of DNA–protein complexes

I Heller, TP Hoekstra, GA King, EJG Peterman… - Chemical …, 2014 - ACS Publications
Cellular DNA is continuously engaged in a molecular dance with many accessory proteins.
These proteins are responsible for orchestrating the storage, maintenance, and transfer of …

Tip-enhanced Raman imaging of single-stranded DNA with single base resolution

Z He, Z Han, M Kizer, RJ Linhardt, X Wang… - Journal of the …, 2018 - ACS Publications
Tip-enhanced Raman scattering (TERS) is a promising optical and analytical technique for
chemical imaging and sensing at single molecule resolution. In particular, TERS signals …

New insights into an old story: Agrobacterium‐induced tumour formation in plants by plant transformation

A Pitzschke, H Hirt - The EMBO Journal, 2010 - embopress.org
Agrobacterium tumefaciens causes tumour formation in plants. Plant signals induce in the
bacteria the expression of a range of virulence (Vir) proteins and the formation of a type IV …

Type IV secretion systems: tools of bacterial horizontal gene transfer and virulence

M Juhas, DW Crook, DW Hood - Cellular microbiology, 2008 - Wiley Online Library
Type IV secretion systems (T4SSs) are multisubunit cell‐envelope‐spanning structures,
ancestrally related to bacterial conjugation machines, which transfer proteins and …

Traversing the Cell: Agrobacterium T-DNA's Journey to the Host Genome

SB Gelvin - Frontiers in plant science, 2012 - frontiersin.org
The genus Agrobacterium is unique in its ability to conduct interkingdom genetic exchange.
Virulent Agrobacterium strains transfer single-strand forms of T-DNA (T-strands) and several …

[LLIBRE][B] Plant tissue culture, development, and biotechnology

RN Trigiano, DJ Gray - 2016 - taylorfrancis.com
Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields.
Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different …

The roles of bacterial and host plant factors in Agrobacterium-mediated genetic transformation

B Lacroix, V Citovsky - The International journal of …, 2013 - pmc.ncbi.nlm.nih.gov
The genetic transformation of plants mediated by Agrobacterium tumefaciens represents an
essential tool for both fundamental and applied research in plant biology. For a successful …

Translation and folding of single proteins in real time

F Wruck, A Katranidis, KH Nierhaus, G Büldt… - Proceedings of the …, 2017 - pnas.org
Protein biosynthesis is inherently coupled to cotranslational protein folding. Folding of the
nascent chain already occurs during synthesis and is mediated by spatial constraints …

Single-molecule views of protein movement on single-stranded DNA

T Ha, AG Kozlov, TM Lohman - Annual review of biophysics, 2012 - annualreviews.org
The advent of new technologies allowing the study of single biological molecules continues
to have a major impact on studies of interacting systems as well as enzyme reactions. These …