Nucleic acid amplification technologies (NAATs) have become fundamental tools in
molecular diagnostics, due to their ability to detect small amounts of target molecules. Since …

Nucleic acids, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), are
natural biopolymers composed of nucleotides that store, transmit, and express genetic …

Abnormal expression of miRNAs is often detected in various human cancers. DNAzyme
machines combined with gold nanoparticles (AuNPs) hold promise for detecting specific …

Nucleic acids are considered as perfect programmable materials for cascade signal
amplification and not merely as genetic information carriers. Among them, catalytic hairpin …

Isothermal amplification of nucleic acids is a simple process that rapidly and efficiently
accumulates nucleic acid sequences at constant temperature. Since the early 1990s …

CRISPR-based diagnostics (CRISPR-Dx) has shown great promise in molecular
diagnostics, but its utility in the sensing of microRNA (miRNA) biomarkers is limited by …

The combination of nanostructures with biomolecules leading to the generation of functional
nanosystems holds great promise for biotechnological and biomedical applications. As a …

Catalytic hairpin assembly (CHA) with advantages of high efficiency, flexible design, and
mild reaction condition is considered a promising molecular amplifier. Modified working …

DNAzymes have been recognized as promising transducing agents for visualizing
endogenous biomarkers, but their inefficient intracellular delivery and limited amplification …

MicroRNA (miRNA) imaging in disease sites is vital to elucidate their role in cancer
progression. However, limited tumor specificity remains a major barrier for traditional …