Aquaculture is the fastest-growing food production area in the world and this productive
activity requires nutrients that fit the needs of cultured species, as well as additives or …

In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

Genome-scale metabolic models (GEMs) represent extensive knowledgebases that provide
a platform for model simulations and integrative analysis of omics data. This study …

Methanol, an organic one-carbon (C1) compound, represents an attractive alternative
carbon source for microbial fermentation. Despite considerable advancements in methanol …

Metabolic engineering aims to develop efficient cell factories by rewiring cellular
metabolism. As one of the most commonly used cell factories, Saccharomyces cerevisiae …

Recombinant proteins (RP) are widely used as biopharmaceuticals, industrial enzymes, or
sustainable food source. Yeasts, with their ability to produce complex proteins through a …

This overview guides both novices and experienced researchers facing challenging targets
to select the most appropriate gene expression system for producing a particular protein. By …

Pichia pastoris (syn. Komagataella spp.) has attracted extensive attention as an efficient
platform for recombinant protein (RP) production. For obtaining a higher protein titer, many …

Eukaryotic cells are used as cell factories to produce and secrete multitudes of recombinant
pharmaceutical proteins, including several of the current top-selling drugs. Due to the …

Microbial proteins are promising substitutes for animal-and plant-based proteins. S.
cerevisiae, a generally recognized as safe (GRAS) microorganism, has been frequently …