Surface-induced protein aggregation and particle formation in biologics: current understanding of mechanisms, detection and mitigation strategies
Protein stability against aggregation is a major quality concern for the production of safe and
effective biopharmaceuticals. Amongst the different drivers of protein aggregation …
effective biopharmaceuticals. Amongst the different drivers of protein aggregation …
[HTML][HTML] A framework for the biophysical screening of antibody mutations targeting solvent-accessible hydrophobic and electrostatic patches for enhanced viscosity …
GB Armstrong, V Shah, P Sanches, M Patel… - Computational and …, 2024 - Elsevier
The formulation of high-concentration monoclonal antibody (mAb) solutions in low dose
volumes for autoinjector devices poses challenges in manufacturability and patient …
volumes for autoinjector devices poses challenges in manufacturability and patient …
Variable domain mutational analysis to probe the molecular mechanisms of high viscosity of an IgG1 antibody
Subcutaneous injection is the preferred route of administration for many antibody
therapeutics for reasons that include its speed and convenience. However, the small volume …
therapeutics for reasons that include its speed and convenience. However, the small volume …
A comparative study of the developability of full-length antibodies, fragments, and bispecific formats reveals higher stability risks for engineered constructs
I Condado-Morales, F Dingfelder, I Waibel… - MAbs, 2024 - Taylor & Francis
Engineered antibody formats, such as antibody fragments and bispecifics, have the potential
to offer improved therapeutic efficacy compared to traditional full-length monoclonal …
to offer improved therapeutic efficacy compared to traditional full-length monoclonal …
A first insight into the developability of an immunoglobulin G3: a combined computational and experimental approach
GB Armstrong, A Lewis, V Shah, P Taylor… - ACS Pharmacology & …, 2024 - ACS Publications
Immunoglobulin G 3 (IgG3) monoclonal antibodies (mAbs) are high-value scaffolds for
develo** novel therapies. Despite their wide-ranging therapeutic potential, IgG3 …
develo** novel therapies. Despite their wide-ranging therapeutic potential, IgG3 …
Biomolecular condensates sustain pH gradients at equilibrium driven by charge neutralisation
Electrochemical gradients are essential to the functioning of cells and are typically formed
across membranes using active transporters and require energy input to maintain them …
across membranes using active transporters and require energy input to maintain them …
Development of Zalfermin, a Long-Acting Proteolytically Stabilized FGF21 Analog
K Sass-Ørum, TM Tagmose, J Olsen… - Journal of Medicinal …, 2024 - ACS Publications
Here, we describe the development of the FGF21 analog zalfermin (NNC0194-0499, 15),
intended for once-weekly sc dosing. Protein engineering was needed to address inherent …
intended for once-weekly sc dosing. Protein engineering was needed to address inherent …
Nonspecificity fingerprints for clinical-stage antibodies in solution
Monoclonal antibodies (mAbs) have successfully been developed for the treatment of a wide
range of diseases. The clinical success of mAbs does not solely rely on optimal potency and …
range of diseases. The clinical success of mAbs does not solely rely on optimal potency and …
AF4 and PEG Precipitation as Predictive Assays for Antibody Self-Association
I Condado-Morales, V Sokolova… - Molecular …, 2023 - ACS Publications
Monoclonal antibodies (mAbs) are often formulated as high-protein-concentration solutions,
which in some cases can exhibit physical stability issues such as high viscosity and …
which in some cases can exhibit physical stability issues such as high viscosity and …
A first insight into the developability of an IgG3: A combined computational and experimental approach
ABSTRACT Immunoglobulin G 3 (IgG3) monoclonal antibodies (mAbs) are high value
scaffolds for develo** novel therapies. Despite their wide-ranging therapeutic potential …
scaffolds for develo** novel therapies. Despite their wide-ranging therapeutic potential …