Polarization microscopy: from ensemble structural imaging to single-molecule 3D orientation and localization microscopy
S Brasselet, MA Alonso - Optica, 2023 - opg.optica.org
Optical contrasts in microscopy are sensitive to light polarization, whose interaction with
molecular dipoles provides an important lever for probing molecular orientation. Polarization …
molecular dipoles provides an important lever for probing molecular orientation. Polarization …
Super-resolution imaging: when biophysics meets nanophotonics
Probing light–matter interaction at the nanometer scale is one of the most fascinating topics
of modern optics. Its importance is underlined by the large span of fields in which such …
of modern optics. Its importance is underlined by the large span of fields in which such …
Simultaneous orientation and 3D localization microscopy with a Vortex point spread function
Estimating the orientation and 3D position of rotationally constrained emitters with
localization microscopy typically requires polarization splitting or a large engineered Point …
localization microscopy typically requires polarization splitting or a large engineered Point …
4polar-STORM polarized super-resolution imaging of actin filament organization in cells
Single-molecule localization microscopy provides insights into the nanometer-scale spatial
organization of proteins in cells, however it does not provide information on their …
organization of proteins in cells, however it does not provide information on their …
Single-molecule orientation localization microscopy I: fundamental limits
Precisely measuring the three-dimensional position and orientation of individual
fluorophores is challenging due to the substantial photon shot noise in single-molecule …
fluorophores is challenging due to the substantial photon shot noise in single-molecule …
Robust and bias-free localization of individual fixed dipole emitters achieving the Cramér Rao bound for applications in cryo-single molecule localization microscopy
Single molecule localization microscopy (SMLM) has the potential to resolve structural
details of biological samples at the nanometer length scale. Compared to room temperature …
details of biological samples at the nanometer length scale. Compared to room temperature …
Fluorescence Interference-Based Polarized Structured Illumination Microscopy for High Axial Accuracy Morphology Imaging of Dipole Orientations
The dipole orientation of fluorescent molecules reveals the structural organization at the
subcellular level, and imaging its distribution is important for fundamental cell biology …
subcellular level, and imaging its distribution is important for fundamental cell biology …
Combining single-molecule super-resolved localization microscopy with fluorescence polarization imaging to study cellular processes
Super-resolution microscopy has catalyzed valuable insights into the sub-cellular,
mechanistic details of many different biological processes across a wide range of cell types …
mechanistic details of many different biological processes across a wide range of cell types …
Linearly polarized excitation enhances signals from fluorescent voltage indicators
Voltage imaging in cells requires high-speed recording of small fluorescent signals, often
leading to low signal/noise ratios. Because voltage indicators are membrane bound, their …
leading to low signal/noise ratios. Because voltage indicators are membrane bound, their …
Three-dimensional single molecule localization close to the coverslip: a comparison of methods exploiting supercritical angle fluorescence
The precise spatial localization of single molecules in three dimensions is an important
basis for single molecule localization microscopy (SMLM) and tracking. At distances up to a …
basis for single molecule localization microscopy (SMLM) and tracking. At distances up to a …