Click chemistry in proteomic investigations
Despite advances in genetic and proteomic techniques, a complete portrait of the proteome
and its complement of dynamic interactions and modifications remains a lofty, and as of yet …
and its complement of dynamic interactions and modifications remains a lofty, and as of yet …
Activity‐based sensing: a synthetic methods approach for selective molecular imaging and beyond
Emerging from the origins of supramolecular chemistry and the development of selective
chemical receptors that rely on lock‐and‐key binding, activity‐based sensing (ABS)—which …
chemical receptors that rely on lock‐and‐key binding, activity‐based sensing (ABS)—which …
Introducing a covalent thiol-based protected immobilized acetylcholinesterase with enhanced enzymatic performances for biosynthesis of esters
SRH Jangi, M Akhond - Process Biochemistry, 2022 - Elsevier
Acetylcholinesterase covalent immobilization was performed on mercaptoacetic acid-
functionalized magnetic nanoparticles. Upon the thiol-based protection of the enzyme active …
functionalized magnetic nanoparticles. Upon the thiol-based protection of the enzyme active …
Target validation using chemical probes
Fully profiled chemical probes are essential to support the unbiased interpretation of
biological experiments necessary for rigorous preclinical target validation. We believe that …
biological experiments necessary for rigorous preclinical target validation. We believe that …
Enzyme inhibitor discovery by activity-based protein profiling
MJ Niphakis, BF Cravatt - Annual review of biochemistry, 2014 - annualreviews.org
Eukaryotic and prokaryotic organisms possess huge numbers of uncharacterized enzymes.
Selective inhibitors offer powerful probes for assigning functions to enzymes in native …
Selective inhibitors offer powerful probes for assigning functions to enzymes in native …
ABHD17 proteins are novel protein depalmitoylases that regulate N-Ras palmitate turnover and subcellular localization
DTS Lin, E Conibear - elife, 2015 - elifesciences.org
Dynamic changes in protein S-palmitoylation are critical for regulating protein localization
and signaling. Only two enzymes-the acyl-protein thioesterases APT1 and APT2–are known …
and signaling. Only two enzymes-the acyl-protein thioesterases APT1 and APT2–are known …
The metabolic serine hydrolases and their functions in mammalian physiology and disease
Serine hydrolases (SHs) consist of> 200 enzymes in humans characterized by the presence
of an active site serine that is used for the hydrolysis of substrates. The membership of this …
of an active site serine that is used for the hydrolysis of substrates. The membership of this …
Chemical probes of endocannabinoid metabolism
The endocannabinoid signaling system regulates diverse physiologic processes and has
attracted considerable attention as a potential pharmaceutical target for treating diseases …
attracted considerable attention as a potential pharmaceutical target for treating diseases …
Metabolism of the endocannabinoid anandamide: open questions after 25 years
M Maccarrone - Frontiers in molecular neuroscience, 2017 - frontiersin.org
Cannabis extracts have been used for centuries, but its main active principle∆ 9-
tetrahydrocannabinol (THC) was identified about 50 years ago. Yet, it is only 25 years ago …
tetrahydrocannabinol (THC) was identified about 50 years ago. Yet, it is only 25 years ago …
Covalent protein modification: the current landscape of residue-specific electrophiles
DA Shannon, E Weerapana - Current opinion in chemical biology, 2015 - Elsevier
Highlights•Electrophiles can show distinct amino-acid selectivities in proteomes.•The
judicious selection of an electrophile is critical to various chemical-proteomic applications.•A …
judicious selection of an electrophile is critical to various chemical-proteomic applications.•A …