Microalgae as a future food source

Y Torres-Tiji, FJ Fields, SP Mayfield - Biotechnology advances, 2020 - Elsevier
One of the key challenges that we face in the 21st century is the need to feed an ever-
increasing human population with increasingly limited natural resources. Even today it is …

Microfluidics and surface-enhanced Raman spectroscopy, a win–win combination?

R Panneerselvam, H Sadat, EM Höhn, A Das… - Lab on a Chip, 2022 - pubs.rsc.org
With the continuous development in nanoscience and nanotechnology, analytical
techniques like surface-enhanced Raman spectroscopy (SERS) render structural and …

An automated Raman-based platform for the sorting of live cells by functional properties

KS Lee, M Palatinszky, FC Pereira, J Nguyen… - Nature …, 2019 - nature.com
Stable-isotope probing is widely used to study the function of microbial taxa in their natural
environment, but sorting of isotopically labelled microbial cells from complex samples for …

Genome editing of model oleaginous microalgae Nannochloropsis spp. by CRISPR/Cas9

Q Wang, Y Lu, Y **n, L Wei, S Huang, J Xu - The Plant Journal, 2016 - Wiley Online Library
Microalgae are promising feedstock for biofuels yet mechanistic probing of their cellular
network and industrial strain development have been hindered by lack of genome‐editing …

Microfluidic technologies for circulating tumor cell isolation

H Cho, J Kim, H Song, KY Sohn, MH Jeon, KH Han - Analyst, 2018 - pubs.rsc.org
Metastasis is the main cause of tumor-related death, and the dispersal of tumor cells through
the circulatory system is a critical step in the metastatic process. Early detection and analysis …

Recent advances in the use of microfluidic technologies for single cell analysis

TW Murphy, Q Zhang, LB Naler, S Ma, C Lu - Analyst, 2018 - pubs.rsc.org
The inherent heterogeneity in cell populations has become of great interest and importance
as analytical techniques have improved over the past decades. With the advent of …

Metabolic-Activity-Based Assessment of Antimicrobial Effects by D2O-Labeled Single-Cell Raman Microspectroscopy

Y Tao, Y Wang, S Huang, P Zhu, WE Huang… - Analytical …, 2017 - ACS Publications
To combat the spread of antibiotic resistance, methods that quantitatively assess the
metabolism-inhibiting effects of drugs in a rapid and culture-independent manner are …

Raman-activated droplet sorting (RADS) for label-free high-throughput screening of microalgal single-cells

X Wang, L Ren, Y Su, Y Ji, Y Liu, C Li, X Li… - Analytical …, 2017 - ACS Publications
Raman-activated cell sorting (RACS) has attracted increasing interest, yet throughput
remains one major factor limiting its broader application. Here we present an integrated …

Robust spontaneous Raman flow cytometry for single‐cell metabolic phenome profiling via pDEP‐DLD‐RFC

X Wang, L Ren, Z Diao, Y He, J Zhang, M Liu… - Advanced …, 2023 - Wiley Online Library
A full‐spectrum spontaneous single‐cell Raman spectrum (fs‐SCRS) captures the metabolic
phenome for a given cellular state of the cell in a label‐free, landscape‐like manner. Herein …

Fluorescence in situ hybridization (FISH) and cell sorting of living bacteria

G Batani, K Bayer, J Böge, U Hentschel, T Thomas - Scientific reports, 2019 - nature.com
Despite the development of several cultivation methods, the rate of discovery of
microorganisms that are yet-to-be cultivated outpaces the rate of isolating and cultivating …