In the past 15 years, there have been rapid developments in the ability to cotranslationally
incorporate unnatural amino acids into proteins produced in cells, both at defined sites via …

The extracellular matrix (ECM) constitutes the main acellular microenvironment of cells in
almost all tissues and organs. The ECM not only provides mechanical support, but also …

Over the last decade, the ability to genetically encode unnatural amino acids (UAAs) has
evolved rapidly. The programmed incorporation of UAAs into recombinant proteins relies on …

Puromycin is a naturally occurring aminonucleoside antibiotic that inhibits protein synthesis
by ribosome-catalyzed incorporation into the C-terminus of elongating nascent chains …

Do young and old protein molecules have the same probability to be degraded? We
addressed this question using metabolic pulse-chase labeling and quantitative mass …

Chemical modification of proteins is an important tool for probing natural systems, creating
therapeutic conjugates and generating novel protein constructs. Site-selective reactions …

In all cells, proteins are continuously synthesized and degraded to maintain protein
homeostasis and modify gene expression levels in response to stimuli. Collectively, the …

Unraveling the molecular mechanisms governing long-term synaptic plasticity is a key to
understanding how the brain stores information in neural circuits and adapts to a changing …

Functional tools are needed to understand complex biological systems. Here we review how
chemical reporters in conjunction with bioorthogonal labeling methods can be used to …

Here we describe the application of a new click chemistry method for fluorescent tracking of
protein synthesis in individual microorganisms within environmental samples. This …