Quantitative mass spectrometry-based proteomics: an overview
S Rozanova, K Barkovits, M Nikolov, C Schmidt… - Quantitative methods in …, 2021 - Springer
In recent decades, mass spectrometry has moved more than ever before into the front line of
protein-centered research. After being established at the qualitative level, the more …
protein-centered research. After being established at the qualitative level, the more …
The Perseus computational platform for comprehensive analysis of (prote) omics data
A main bottleneck in proteomics is the downstream biological analysis of highly multivariate
quantitative protein abundance data generated using mass-spectrometry-based analysis …
quantitative protein abundance data generated using mass-spectrometry-based analysis …
Quantitative proteomics identifies the core proteome of exosomes with syntenin-1 as the highest abundant protein and a putative universal biomarker
Exosomes are extracellular vesicles derived from the endosomal compartment that are
potentially involved in intercellular communication. Here, we found that frequently used …
potentially involved in intercellular communication. Here, we found that frequently used …
Reproducible workflow for multiplexed deep-scale proteome and phosphoproteome analysis of tumor tissues by liquid chromatography–mass spectrometry
Here we present an optimized workflow for global proteome and phosphoproteome analysis
of tissues or cell lines that uses isobaric tags (TMT (tandem mass tags)-10) for multiplexed …
of tissues or cell lines that uses isobaric tags (TMT (tandem mass tags)-10) for multiplexed …
Perseus: a bioinformatics platform for integrative analysis of proteomics data in cancer research
Mass spectrometry-based proteomics is a continuously growing field marked by
technological and methodological improvements. Cancer proteomics is aimed at pursuing …
technological and methodological improvements. Cancer proteomics is aimed at pursuing …
Robust dimethyl‐based multiplex‐DIA doubles single‐cell proteome depth via a reference channel
Single‐cell proteomics aims to characterize biological function and heterogeneity at the
level of proteins in an unbiased manner. It is currently limited in proteomic depth, throughput …
level of proteins in an unbiased manner. It is currently limited in proteomic depth, throughput …
Global, quantitative and dynamic map** of protein subcellular localization
Subcellular localization critically influences protein function, and cells control protein
localization to regulate biological processes. We have developed and applied Dynamic …
localization to regulate biological processes. We have developed and applied Dynamic …
Protein analysis by shotgun/bottom-up proteomics
According to Genome Sequencing Project statistics (http://www. ncbi. nlm. nih.
gov/genomes/static/gpstat. html), as of February 16, 2012, complete gene sequences have …
gov/genomes/static/gpstat. html), as of February 16, 2012, complete gene sequences have …
[HTML][HTML] Proteomics of melanoma response to immunotherapy reveals mitochondrial dependence
Immunotherapy has revolutionized cancer treatment, yet most patients do not respond. Here,
we investigated mechanisms of response by profiling the proteome of clinical samples from …
we investigated mechanisms of response by profiling the proteome of clinical samples from …
Quantitative proteomics: challenges and opportunities in basic and applied research
In this Perspective, we discuss developments in mass-spectrometry-based proteomic
technology over the past decade from the viewpoint of our laboratory. We also reflect on …
technology over the past decade from the viewpoint of our laboratory. We also reflect on …