Molecular responses to acute exercise and their relevance for adaptations in skeletal muscle to exercise training

B Egan, AP Sharples - Physiological Reviews, 2023 - journals.physiology.org
Repeated, episodic bouts of skeletal muscle contraction undertaken frequently as structured
exercise training are a potent stimulus for physiological adaptation in many organs …

Mechanisms of mechanical overload-induced skeletal muscle hypertrophy: current understanding and future directions

MD Roberts, JJ McCarthy… - Physiological …, 2023 - journals.physiology.org
Mechanisms underlying mechanical overload-induced skeletal muscle hypertrophy have
been extensively researched since the landmark report by Morpurgo (1897) of “work …

Advanced glycation end products (AGEs): biochemistry, signaling, analytical methods, and epigenetic effects

A Perrone, A Giovino, J Benny… - Oxidative medicine and …, 2020 - Wiley Online Library
The advanced glycation end products (AGEs) are organic molecules formed in any living
organisms with a great variety of structural and functional properties. They are considered …

Transfection types, methods and strategies: a technical review

ZX Chong, SK Yeap, WY Ho - PeerJ, 2021 - peerj.com
Transfection is a modern and powerful method used to insert foreign nucleic acids into
eukaryotic cells. The ability to modify host cells' genetic content enables the broad …

An Overview of Methods and Tools for Transfection of Eukaryotic Cells in vitro

A Fus-Kujawa, P Prus, K Bajdak-Rusinek… - … in bioengineering and …, 2021 - frontiersin.org
Transfection is a powerful analytical tool enabling studies of gene products and functions in
eukaryotic cells. Successful delivery of genetic material into cells depends on DNA quantity …

Hyperglycemia, hydroxychloroquine, and the COVID‐19 pandemic

A Brufsky - Journal of medical virology, 2020 - Wiley Online Library
Abstract Coronavirus disease‐2019 (COVID‐19) infection and its severity can be explained
by the concentration of glycosylated severe acute respiratory syndrome‐coronavirus 2 …

SON and SRRM2 are essential for nuclear speckle formation

İA Ilik, M Malszycki, AK Lübke, C Schade, D Meierhofer… - elife, 2020 - elifesciences.org
Nuclear speckles (NS) are among the most prominent biomolecular condensates. Despite
their prevalence, research on the function of NS is virtually restricted to colocalization …

[HTML][HTML] A systematic approach to quantitative Western blot analysis

L Pillai-Kastoori, AR Schutz-Geschwender… - Analytical …, 2020 - Elsevier
Attaining true quantitative data from WB requires that all the players involved in the
procedure are quality controlled including the user. Appropriate protein extraction method …

Protein purification and analysis: next generation Western blotting techniques

M Mishra, S Tiwari, AV Gomes - Expert review of proteomics, 2017 - Taylor & Francis
Introduction: Western blotting is one of the most commonly used techniques in molecular
biology and proteomics. Since western blotting is a multistep protocol, variations and errors …

[HTML][HTML] Proteomes are of proteoforms: embracing the complexity

K Carbonara, M Andonovski, JR Coorssen - Proteomes, 2021 - mdpi.com
Proteomes are complex—much more so than genomes or transcriptomes. Thus, simplifying
their analysis does not simplify the issue. Proteomes are of proteoforms, not canonical …