CRISPR technology: A decade of genome editing is only the beginning

JY Wang, JA Doudna - Science, 2023‏ - science.org
The advent of clustered regularly interspaced short palindromic repeat (CRISPR) genome
editing, coupled with advances in computing and imaging capabilities, has initiated a new …

CRISPR technologies for genome, epigenome and transcriptome editing

L Villiger, J Joung, L Koblan, J Weissman… - … Reviews Molecular Cell …, 2024‏ - nature.com
Our ability to edit genomes lags behind our capacity to sequence them, but the growing
understanding of CRISPR biology and its application to genome, epigenome and …

Past, present, and future of CRISPR genome editing technologies

M Pacesa, O Pelea, M **ek - Cell, 2024‏ - cell.com
Genome editing has been a transformative force in the life sciences and human medicine,
offering unprecedented opportunities to dissect complex biological processes and treat the …

CRISPR/Cas9 therapeutics: progress and prospects

T Li, Y Yang, H Qi, W Cui, L Zhang, X Fu, X He… - Signal transduction and …, 2023‏ - nature.com
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated
protein 9 (Cas9) gene-editing technology is the ideal tool of the future for treating diseases …

Amplification-free CRISPR/Cas detection technology: challenges, strategies, and perspectives

H Li, Y **e, F Chen, H Bai, L **u, X Zhou… - Chemical Society …, 2023‏ - pubs.rsc.org
Rapid and accurate molecular diagnosis is a prerequisite for precision medicine, food
safety, and environmental monitoring. The clustered regularly interspaced short palindromic …

CRISPR in cancer biology and therapy

A Katti, BJ Diaz, CM Caragine, NE Sanjana… - Nature Reviews …, 2022‏ - nature.com
Over the past decade, CRISPR has become as much a verb as it is an acronym,
transforming biomedical research and providing entirely new approaches for dissecting all …

CRISPR-based diagnostics

MM Kaminski, OO Abudayyeh, JS Gootenberg… - Nature biomedical …, 2021‏ - nature.com
The accurate and timely diagnosis of disease is a prerequisite for efficient therapeutic
intervention and epidemiological surveillance. Diagnostics based on the detection of nucleic …

A review on the mechanism and applications of CRISPR/Cas9/Cas12/Cas13/Cas14 proteins utilized for genome engineering

VE Hillary, SA Ceasar - Molecular biotechnology, 2023‏ - Springer
The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-
associated protein (CRISPR/Cas) system has altered life science research offering …

Assessing and advancing the safety of CRISPR-Cas tools: from DNA to RNA editing

J Tao, DE Bauer, R Chiarle - Nature Communications, 2023‏ - nature.com
CRISPR-Cas gene editing has revolutionized experimental molecular biology over the past
decade and holds great promise for the treatment of human genetic diseases. Here we …

Fast and sensitive detection of SARS-CoV-2 RNA using suboptimal protospacer adjacent motifs for Cas12a

S Lu, X Tong, Y Han, K Zhang, Y Zhang… - Nature biomedical …, 2022‏ - nature.com
CRISPR-based assays for the detection of nucleic acids are highly specific, yet they are not
fast, sensitive or easy to use. Here we report a one-step fluorescence assay for the detection …