Brain organoids: advances, applications and challenges

X Qian, H Song, G Ming - Development, 2019 - journals.biologists.com
Brain organoids are self-assembled three-dimensional aggregates generated from
pluripotent stem cells with cell types and cytoarchitectures that resemble the embryonic …

The use of brain organoids to investigate neural development and disease

E Di Lullo, AR Kriegstein - Nature Reviews Neuroscience, 2017 - nature.com
Understanding the development and dysfunction of the human brain is a major goal of
neurobiology. Much of our current understanding of human brain development has been …

[HTML][HTML] Single-cell epigenomics reveals mechanisms of human cortical development

RS Ziffra, CN Kim, JM Ross, A Wilfert, TN Turner… - Nature, 2021 - nature.com
During mammalian development, differences in chromatin state coincide with cellular
differentiation and reflect changes in the gene regulatory landscape. In the develo** brain …

Cell stress in cortical organoids impairs molecular subtype specification

A Bhaduri, MG Andrews, W Mancia Leon, D Jung… - Nature, 2020 - nature.com
Cortical organoids are self-organizing three-dimensional cultures that model features of the
develo** human cerebral cortex,. However, the fidelity of organoid models remains …

Spatiotemporal gene expression trajectories reveal developmental hierarchies of the human cortex

TJ Nowakowski, A Bhaduri, AA Pollen, B Alvarado… - Science, 2017 - science.org
Systematic analyses of spatiotemporal gene expression trajectories during organogenesis
have been challenging because diverse cell types at different stages of maturation and …

[HTML][HTML] Chromatin and gene-regulatory dynamics of the develo** human cerebral cortex at single-cell resolution

AE Trevino, F Müller, J Andersen, L Sundaram… - Cell, 2021 - cell.com
Genetic perturbations of cortical development can lead to neurodevelopmental disease,
including autism spectrum disorder (ASD). To identify genomic regions crucial to …

Proper acquisition of cell class identity in organoids allows definition of fate specification programs of the human cerebral cortex

A Uzquiano, AJ Kedaigle, M Pigoni, B Paulsen… - Cell, 2022 - cell.com
Realizing the full utility of brain organoids to study human development requires
understanding whether organoids precisely replicate endogenous cellular and molecular …

Human brain organogenesis: Toward a cellular understanding of development and disease

KW Kelley, SP Pașca - Cell, 2022 - cell.com
The construction of the human nervous system is a distinctly complex although highly
regulated process. Human tissue inaccessibility has impeded a molecular understanding of …

New insights into the development of the human cerebral cortex

Z Molnár, GJ Clowry, N Šestan, A Alzu'bi… - Journal of …, 2019 - Wiley Online Library
The cerebral cortex constitutes more than half the volume of the human brain and is
presumed to be responsible for the neuronal computations underlying complex phenomena …

Human iPSC-derived cerebral organoids model cellular features of lissencephaly and reveal prolonged mitosis of outer radial glia

M Bershteyn, TJ Nowakowski, AA Pollen, E Di Lullo… - Cell stem cell, 2017 - cell.com
Classical lissencephaly is a genetic neurological disorder associated with mental
retardation and intractable epilepsy, and Miller-Dieker syndrome (MDS) is the most severe …