Segregation of the replicated genome during cell division in eukaryotes requires the
kinetochore to link centromeric DNA to spindle microtubules. The kinetochore is composed …

In eukaryotic cells, the spatial segregation of replication and transcription in the nucleus and
translation in the cytoplasm imposes the requirement of transporting thousands of …

INTRODUCTION Cytoplasmic face of the human NPC. Near-atomic composite structure of
the NPC generated by docking high-resolution crystal structures into a cryo‑ET …

Proximity-dependent biotin identification (BioID) is a method for identifying protein
associations that occur in vivo. By fusing a promiscuous biotin ligase to a protein of interest …

The BioID method uses a promiscuous biotin ligase to detect protein–protein associations
as well as proximate proteins in living cells. Here we report improvements to the BioID …

Cell division in eukaryotes requires extensive architectural changes of the nuclear envelope
(NE) to ensure that segregated DNA is finally enclosed in a single cell nucleus in each …

Nuclear pore complexes (NPCs) are indispensable for cell function and are at the center of
several human diseases. NPCs provide access to the nucleus and regulate the transport of …

The nuclear envelope (NE) is a highly regulated membrane barrier that separates the
nucleus from the cytoplasm in eukaryotic cells. It contains a large number of different …

Nuclear pore complexes are large aqueous channels that penetrate the nuclear envelope,
thereby connecting the nuclear interior with the cytoplasm. Until recently, these …

The nuclear pore complex (NPC) mediates nucleo-cytoplasmic transport of macromolecules
and is an obligatory point of passage and functional bottleneck in the replication of some …