Cryo-electron tomography on focused ion beam lamellae transforms structural cell biology
Cryogenic electron microscopy and data processing enable the determination of structures
of isolated macromolecules to near-atomic resolution. However, these data do not provide …
of isolated macromolecules to near-atomic resolution. However, these data do not provide …
The promise and the challenges of cryo‐electron tomography
M Turk, W Baumeister - FEBS letters, 2020 - Wiley Online Library
Structural biologists have traditionally approached cellular complexity in a reductionist
manner in which the cellular molecular components are fractionated and purified before …
manner in which the cellular molecular components are fractionated and purified before …
Isotropic reconstruction for electron tomography with deep learning
Cryogenic electron tomography (cryoET) allows visualization of cellular structures in situ.
However, anisotropic resolution arising from the intrinsic “missing-wedge” problem has …
However, anisotropic resolution arising from the intrinsic “missing-wedge” problem has …
[HTML][HTML] Implementation of a cryo-electron tomography tilt-scheme optimized for high resolution subtomogram averaging
Cryo-electron tomography (cryoET) allows 3D structural information to be obtained from
cells and other biological samples in their close-to-native state. In combination with …
cells and other biological samples in their close-to-native state. In combination with …
Bringing structure to cell biology with cryo-electron tomography
Recent advances in cryo-electron microscopy have marked only the beginning of the
potential of this technique. To bring structure into cell biology, the modality of cryo-electron …
potential of this technique. To bring structure into cell biology, the modality of cryo-electron …
Convolutional networks for supervised mining of molecular patterns within cellular context
I de Teresa-Trueba, SK Goetz, A Mattausch… - Nature …, 2023 - nature.com
Cryo-electron tomograms capture a wealth of structural information on the molecular
constituents of cells and tissues. We present DeePiCt (deep picker in context), an open …
constituents of cells and tissues. We present DeePiCt (deep picker in context), an open …
The enigma of the respiratory chain supercomplex
Respiratory chain dysfunction plays an important role in human disease and aging. It is now
well established that the individual respiratory complexes can be organized into …
well established that the individual respiratory complexes can be organized into …
TomoTwin: generalized 3D localization of macromolecules in cryo-electron tomograms with structural data mining
G Rice, T Wagner, M Stabrin, O Sitsel, D Prumbaum… - Nature …, 2023 - nature.com
Cryogenic-electron tomography enables the visualization of cellular environments in
extreme detail, however, tools to analyze the full amount of information contained within …
extreme detail, however, tools to analyze the full amount of information contained within …
High-resolution in situ structure determination by cryo-electron tomography and subtomogram averaging using emClarity
Cryo-electron tomography and subtomogram averaging (STA) has developed rapidly in
recent years. It provides structures of macromolecular complexes in situ and in cellular …
recent years. It provides structures of macromolecular complexes in situ and in cellular …
Membranes under the magnetic lens: a dive into the diverse world of membrane protein structures using Cryo-EM
Membrane proteins are highly diverse in both structure and function and can, therefore,
present different challenges for structure determination. They are biologically important for …
present different challenges for structure determination. They are biologically important for …