Single-molecule localization microscopy

M Lelek, MT Gyparaki, G Beliu, F Schueder… - Nature reviews …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

[HTML][HTML] Challenges and opportunities in exosome research—Perspectives from biology, engineering, and cancer therapy

X Li, AL Corbett, E Taatizadeh, N Tasnim, JP Little… - APL …, 2019 - pubs.aip.org
Exosomes are small (∼ 30–140 nm) lipid bilayer-enclosed particles of endosomal origin.
They are a subset of extracellular vesicles (EVs) that are secreted by most cell types. There …

Membrane phase separation drives responsive assembly of receptor signaling domains

SA Shelby, I Castello-Serrano, KC Wisser… - Nature Chemical …, 2023 - nature.com
Plasma membrane heterogeneity has been tied to a litany of cellular functions and is often
explained by analogy to membrane phase separation; however, models based on phase …

Targeted solvatochromic fluorescent probes for imaging lipid order in organelles under oxidative and mechanical stress

DI Danylchuk, PH Jouard… - Journal of the American …, 2021 - ACS Publications
Biomembranes constitute a basis for all compartments of live cells, and therefore, the
monitoring of their lipid organization is essential for understanding cell status and activity …

Surface-confined electrochemiluminescence microscopy of cell membranes

S Voci, B Goudeau, G Valenti, A Lesch… - Journal of the …, 2018 - ACS Publications
Herein is reported a surface-confined microscopy based on electrochemiluminescence
(ECL) that allows to image the plasma membrane of single cells at the interface with an …

Super-resolution microscopy with single molecules in biology and beyond–essentials, current trends, and future challenges

L Möckl, WE Moerner - Journal of the American Chemical …, 2020 - ACS Publications
Single-molecule super-resolution microscopy has developed from a specialized technique
into one of the most versatile and powerful imaging methods of the nanoscale over the past …

Six-dimensional single-molecule imaging with isotropic resolution using a multi-view reflector microscope

O Zhang, Z Guo, Y He, T Wu, MD Vahey, MD Lew - Nature photonics, 2023 - nature.com
Imaging of both the positions and orientations of single fluorophores, termed single-
molecule orientation-localization microscopy, is a powerful tool for the study of biochemical …

Switchable solvatochromic probes for live‐cell super‐resolution imaging of plasma membrane organization

DI Danylchuk, S Moon, K Xu… - Angewandte …, 2019 - Wiley Online Library
Visualization of the nanoscale organization of cell membranes remains challenging
because of the lack of appropriate fluorescent probes. Herein, we introduce a new design …

Protein sorting by lipid phase-like domains supports emergent signaling function in B lymphocyte plasma membranes

MB Stone, SA Shelby, MF Núñez, K Wisser, SL Veatch - elife, 2017 - elifesciences.org
Diverse cellular signaling events, including B cell receptor (BCR) activation, are
hypothesized to be facilitated by domains enriched in specific plasma membrane lipids and …

Advanced functional fluorescent probes for cell plasma membranes

M Collot, S Pfister, AS Klymchenko - Current Opinion in Chemical Biology, 2022 - Elsevier
Imaging the plasma membrane (PM) by fluorescence techniques using molecular
fluorescent probes enable cell segmentation, studying membrane organization and …