Comparison of destructive and nondestructive DNA extraction methods for the metabarcoding of arthropod bulk samples

A Kirse, SJ Bourlat, K Langen, B Zapke… - Molecular Ecology …, 2023 - Wiley Online Library
DNA metabarcoding is routinely used for biodiversity assessment, in particular targeting
highly diverse groups for which limited taxonomic expertise is available. Various protocols …

DNA metabarcoding and morphological identification reveal similar richness, taxonomic composition and body size patterns among flying insect communities

N Remmel, D Buchner, J Enss… - Insect Conservation …, 2024 - Wiley Online Library
Tracking insect biodiversity trends and predicting future trajectories is limited by a lack of
monitoring data across large spatiotemporal scales. DNA metabarcoding is a time‐and cost …

Upscaling biodiversity monitoring: Metabarcoding estimates 31,846 insect species from Malaise traps across Germany

D Buchner, JS Sinclair, M Ayasse… - Molecular Ecology …, 2025 - Wiley Online Library
Mitigating ongoing losses of insects and their key functions (eg pollination) requires tracking
large‐scale and long‐term community changes. However, doing so has been hindered by …

FAVIS: Fast and versatile protocol for non-destructive metabarcoding of bulk insect samples

E Iwaszkiewicz-Eggebrecht, P Łukasik, M Buczek… - PloS one, 2023 - journals.plos.org
Insects are diverse and sustain essential ecosystem functions, yet remain understudied.
Recent reports about declines in insect abundance and diversity have highlighted a …

Recommendations for effective insect conservation in nature protected areas based on a transdisciplinary project in Germany

S Köthe, N Bakanov, CA Brühl, L Eichler… - Environmental Sciences …, 2023 - Springer
The decline of insect abundance and richness has been documented for decades and has
received increased attention in recent years. In 2017, a study by Hallmann and colleagues …

[HTML][HTML] Another crack in the Dark Taxa wall: a custom DNA barcoding protocol for the species-rich and common Eurytomidae (Hymenoptera, Chalcidoidea)

S Jafari, B Müller, B Rulik, V Rduch… - Biodiversity Data …, 2023 - ncbi.nlm.nih.gov
DNA barcodes are a great tool for accelerated species identification and for complementing
species delimitation. Furthermore, DNA barcode reference libraries are the decisive …

Optimized DNA isolation from marine sponges for natural sampler DNA metabarcoding

LR Harper, EF Neave, GS Sellers… - Environmental …, 2023 - Wiley Online Library
Marine sponges have recently been recognized as natural samplers of environmental DNA
(eDNA) due to their effective water filtration and their ubiquitous, sessile, and regenerative …

Differential Mitochondrial Genome Expression of Three Sympatric Lizards in Response to Low-Temperature Stress

J He, L Zhan, S Meng, Z Wang, L Gao, W Wang… - Animals, 2024 - mdpi.com
Simple Summary Global climate change is a growing threat to the lives and ranges of reptile
species. The present study examines three lizard species (Calotes versicolor …

The clockwork of insect activity: Advancing ecological understanding through automation

A Kirse, MA Wittenhorst, C Scherber… - Journal of Animal …, 2025 - Wiley Online Library
Understanding insect behaviour and its underlying drivers is vital for interpreting changes in
local biodiversity and predicting future trends. Conventional insect traps are typically limited …

[PDF][PDF] Homogenization of insect bulk samples yields more comprehensive yet comparable biodiversity data than non-destructive lysis

L Wolany, J Enss, F Leese… - Metabarcoding and …, 2024 - mbmg.pensoft.net
The rapid advancement of molecular biodiversity monitoring tools, particularly DNA
metabarcoding, has improved specimen identification in bulk samples, such as those from …