▪ Abstract In vitro selection allows rare functional RNA or DNA molecules to be isolated from
pools of over 1015 different sequences. This approach has been used to identify RNA and …

Metabolic and signaling biochemical pathways have numerous steps that involve the
hydrolytic cleavage of peptide or phosphate ester bonds. Although both types of bonds are …

An in vitro selection procedure was used to develop a DNA enzyme that can be made to
cleave almost any targeted RNA substrate under simulated physiological conditions. The …

An iterative in vitro selection procedure was used to isolate a new class of catalytic RNAs
(ribozymes) from a large pool of random-sequence RNA molecules. These ribozymes ligate …

Using fluorescence microscopy, we studied the catalysis by and folding of individual
Tetrahymena thermophila ribozyme molecules. The dye-labeled and surface-immobilized …

RNA performs important cellular functions in contemporary life forms. Its ability to act both as
a catalyst and a storage mechanism for genetic information is also an important part of the …

We previously reported the in vitro selection of a general-purpose RNA-cleaving DNA
enzyme that exhibits a catalytic efficiency (k cat/KM) exceeding that of any other known …

▪ Abstract The past few years have seen exciting advances in understanding the structure
and function of catalytic RNA. Crystal structures of several ribozymes have provided detailed …

The group I self-splicing introns, like other RNAs and RNA–protein complexes, undergo
multiple conformational changes in completing two transesterification reactions that cleave …

An in vitro evolution procedure was used to obtain RNA enzymes with a particular catalytic
function. A population of 1013 variants of the Tetrahymena ribozyme, a group I ribozyme that …