Beyond the marks: reader-effectors as drivers of epigenetics and chromatin engineering

KA Franklin, CE Shields, KA Haynes - Trends in biochemical sciences, 2022 - cell.com
Chromatin is a system of proteins and DNA that regulates chromosome organization and
gene expression in eukaryotes. Essential features that support these processes include …

Manipulating cation− π interactions of reader proteins in living cells with genetic code expansion

H Zhao, L Tang, Y Fang, C Liu, W Ding… - Journal of the …, 2023 - ACS Publications
Despite tremendous success in understanding the chemical nature and the importance of
cation− π interactions in a range of biological processes, particularly in epigenetic …

Interactions with histone H3 & tools to study them

WA Scott, EI Campos - Frontiers in Cell and Developmental Biology, 2020 - frontiersin.org
Histones are an integral part of chromatin and thereby influence its structure, dynamics, and
functions. The effects of histone variants, posttranslational modifications, and binding …

Single-electron transfer between sulfonium and tryptophan enables site-selective photo crosslinking of methyllysine reader proteins

F Feng, Y Gao, Q Zhao, T Luo, Q Yang, N Zhao… - Nature Chemistry, 2024 - nature.com
The identification of readers, an important class of proteins that recognize modified residues
at specific sites, is essential to uncover the biological roles of post-translational …

High-affinity chromodomains engineered for improved detection of histone methylation and enhanced CRISPR-based gene repression

G Veggiani, R Villaseñor, GD Martyn, JQ Tang… - Nature …, 2022 - nature.com
Histone methylation is an important post-translational modification that plays a crucial role in
regulating cellular functions, and its dysregulation is implicated in cancer and …

Contribution of Electrostatic CH3–π Interactions to Recognition of Histone Asymmetric Dimethylarginine by the SPIN1 Triple Tudor Domain

CR Travis, RG Dumais, JW Treacy… - Journal of the …, 2024 - ACS Publications
Methylation of arginine (Arg) residues on histones creates a new binding epitope, enabling
recognition by aromatic cage binding pockets in Tudor domains; these protein–protein …

Scalable synthesis of sulfonium and selenonium peptides for selective methyllysine reader crosslinking

Y Gao, F Feng, M Wu - Tetrahedron Letters, 2024 - Elsevier
Lysine methylation readers are an important class of proteins that bind site-specifically
methylated proteins for downstream regulation. Chemical probes that crosslink lysine …

Advances and opportunities in epigenetic chemical biology

JN Beyer, NR Raniszewski, GM Burslem - ChemBioChem, 2021 - Wiley Online Library
The study of epigenetics has greatly benefited from the development and application of
various chemical biology approaches. In this review, we highlight the key targets for …

Selective Triazenation Reaction (STaR) of secondary amines for tagging monomethyl lysine post‐translational modifications

O Nwajiobi, S Mahesh, X Streety, M Raj - Angewandte Chemie, 2021 - Wiley Online Library
Lysine monomethylation (Kme) is an impactful post‐translational modification (PTM)
responsible for regulating biological processes and implicated in diseases, thus there is …

[HTML][HTML] Synthetic Interventions in Epigenome: Unraveling Chromatin's Potential for Therapeutic Applications

J Kim, J Kim, M Park - Current Opinion in Systems Biology, 2024 - Elsevier
The epigenome, comprising DNA and histone modifications alongside intricate chromatin
structures, has emerged as pivotal players in disease development. These factors offer …