Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …

Precise gene editing is—or will soon be—in clinical use for several diseases, and more
applications are under development. The programmable nuclease Cas9, directed by a …

The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …

Rapid, efficient generation of knock-in mice with targeted large insertions remains a major
hurdle in mouse genetics. Here, we describe two-cell homologous recombination (2C-HR) …

The CRISPR-Cas9 system is a powerful genome-editing tool in which a guide RNA targets
Cas9 to a site in the genome, where the Cas9 nuclease then induces a double-stranded …

Key message We summarize recent progress of CRISPR/Cas9-mediated gene targeting in
plants, provide recommendations for designing gene-targeting vectors and highlight the …

Despite the potential of small molecules and recombinant proteins to enhance the efficiency
of homology-directed repair (HDR), single-stranded DNA (ssDNA) donors, as currently …

Writing specific DNA sequences into the human genome is challenging with non-viral gene-
editing reagents, since most of the edited sequences contain various imprecise insertions or …

Our understanding of how genotype controls phenotype is limited by the scale at which we
can precisely alter the genome and assess the phenotypic consequences of each …

Abstract Wheat (Triticum aestivum, 2n= 6x= 42, AABBDD) is one of the most important staple
food crops in the world. Despite the fact that wheat production has significantly increased …