Cell-autonomous effects of APOE4 in restricting microglial response in brain homeostasis and Alzheimer's disease

CC Liu, N Wang, Y Chen, Y Inoue, F Shue, Y Ren… - Nature …, 2023 - nature.com
Microglial involvement in Alzheimer's disease (AD) pathology has emerged as a risk-
determining pathogenic event. While apolipoprotein E (APOE) is known to modify AD risk, it …

Mis-spliced transcripts generate de novo proteins in TDP-43–related ALS/FTD

S Seddighi, YA Qi, AL Brown, OG Wilkins… - Science translational …, 2024 - science.org
Functional loss of TDP-43, an RNA binding protein genetically and pathologically linked to
amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), leads to the …

Development of brain organoid technology derived from iPSC for the neurodegenerative disease modelling: a glance through

AS Jusop, K Thanaskody, GJ Tye, SA Dass… - Frontiers in Molecular …, 2023 - frontiersin.org
Neurodegenerative diseases are adult-onset neurological conditions that are notoriously
difficult to model for drug discovery and development because most models are unable to …

Making gene editing accessible in resource limited environments: recommendations to guide a first-time user

S Goolab, J Scholefield - Frontiers in Genome Editing, 2024 - frontiersin.org
The designer nuclease, CRISPR-Cas9 system has advanced the field of genome
engineering owing to its programmability and ease of use. The application of these …

Map** multimodal phenotypes to perturbations in cells and tissue with CRISPRmap

J Gu, A Iyer, B Wesley, A Taglialatela, G Leuzzi… - Nature …, 2024 - nature.com
Unlike sequencing-based methods, which require cell lysis, optical pooled genetic screens
enable investigation of spatial phenotypes, including cell morphology, protein subcellular …

Disruption of lysosomal proteolysis in astrocytes facilitates midbrain organoid proteostasis failure in an early-onset Parkinson's disease model

G Morrone Parfitt, E Coccia, C Goldman… - Nature …, 2024 - nature.com
Accumulation of advanced glycation end products (AGEs) on biopolymers accompanies
cellular aging and drives poorly understood disease processes. Here, we studied how AGEs …

ApoE4-dependent lysosomal cholesterol accumulation impairs mitochondrial homeostasis and oxidative phosphorylation in human astrocytes

H Lee, S Cho, MJ Kim, YJ Park, E Cho, YS Jo, YS Kim… - Cell Reports, 2023 - cell.com
Recent developments in genome sequencing have expanded the knowledge of genetic
factors associated with late-onset Alzheimer's disease (AD). Among them, genetic variant ε4 …

Dual regulation of SLC25A39 by AFG3L2 and iron controls mitochondrial glutathione homeostasis

X Shi, M DeCiucis, KA Grabinska, J Kanyo, A Liu… - Molecular Cell, 2024 - cell.com
Organelle transporters define metabolic compartmentalization, and how this metabolite
transport process can be modulated is poorly explored. Here, we discovered that human …

Regulatory imbalance between LRRK2 kinase, PPM1H phosphatase, and ARF6 GTPase disrupts the axonal transport of autophagosomes

D Dou, EM Smith, CS Evans, CA Boecker… - Cell reports, 2023 - cell.com
Gain-of-function mutations in the LRRK2 gene cause Parkinson's disease (PD), increasing
phosphorylation of RAB GTPases through hyperactive kinase activity. We find that LRRK2 …

The Foundational Data Initiative for Parkinson Disease: Enabling efficient translation from genetic maps to mechanism

E Bressan, X Reed, V Bansal, E Hutchins, MM Cobb… - Cell genomics, 2023 - cell.com
Summary The Foundational Data Initiative for Parkinson Disease (FOUNDIN-PD) is an
international collaboration producing fundamental resources for Parkinson disease (PD) …