Platelet 12-LOX is essential for FcγRIIa-mediated platelet activation
J Yeung, BE Tourdot… - Blood, The Journal …, 2014 - ashpublications.org
Platelets are essential in maintaining hemostasis following inflammation or injury to the
vasculature. Dysregulated platelet activity often results in thrombotic complications leading …
vasculature. Dysregulated platelet activity often results in thrombotic complications leading …
Cryo-EM structures of human arachidonate 12S-lipoxygenase bound to endogenous and exogenous inhibitors
Abstract Human 12-lipoxygenase (12-LOX) is a key enzyme involved in platelet activation,
and the regulation of its activity has been targeted for the treatment of heparin-induced …
and the regulation of its activity has been targeted for the treatment of heparin-induced …
N-Substituted 5-(1H-Indol-2-yl)-2-methoxyanilines Are Allosteric Inhibitors of the Linoleate Oxygenase Activity of Selected Mammalian ALOX15 Orthologs …
A Golovanov, A Zhuravlev, A Cruz… - Journal of medicinal …, 2022 - ACS Publications
Here, we describe the first systematic study on the mechanism of substrate-selective
inhibition of mammalian ALOX15 orthologs. For this purpose, we prepared a series of N …
inhibition of mammalian ALOX15 orthologs. For this purpose, we prepared a series of N …
[HTML][HTML] Structural and Functional Biology of Mammalian ALOX Isoforms with Particular Emphasis on Enzyme Dimerization and Their Allosteric Properties
A Zhuravlev, V Gavrilyuk, X Chen… - International …, 2024 - pmc.ncbi.nlm.nih.gov
The human genome involves six functional arachidonic acid (AA) lipoxygenase (ALOX)
genes, and the corresponding enzymes (ALOX15, ALOX15B, ALOX12, ALOX12B, ALOXE3 …
genes, and the corresponding enzymes (ALOX15, ALOX15B, ALOX12, ALOX12B, ALOXE3 …
[HTML][HTML] Vitis vinifera lipoxygenase LoxA is an allosteric dimer activated by lipidic surfaces
S Pilati, K Wild, A Gumiero, I Holdermann… - Journal of Molecular …, 2024 - Elsevier
Lipoxygenases catalyze the peroxidation of poly-unsaturated fatty acid chains either free or
esterified in membrane lipids. Vitis vinifera LoxA is transcriptionally induced at ripening …
esterified in membrane lipids. Vitis vinifera LoxA is transcriptionally induced at ripening …
Effect of solvent viscosity on the activation barrier of hydrogen tunneling in the lipoxygenase reaction
L Guevara, M Gouge, A Ohler, SG Hill, S Patel… - Archives of Biochemistry …, 2023 - Elsevier
Hydrogen tunneling in enzyme reactions has played an important role in linking protein
thermal motions to the chemical steps of catalysis. Lipoxygenases (LOXs) have served as …
thermal motions to the chemical steps of catalysis. Lipoxygenases (LOXs) have served as …
[HTML][HTML] Recent Applications of In Silico Approaches for Studying Receptor Mutations Associated with Human Pathologies
M Pappalardo, FM Sipala, MC Nicolosi… - …, 2024 - pmc.ncbi.nlm.nih.gov
In recent years, the advent of computational techniques to predict the potential activity of a
drug interacting with a receptor or to predict the structure of unidentified proteins with …
drug interacting with a receptor or to predict the structure of unidentified proteins with …
Identification of the Thermal Activation Network in Human 15-Lipoxygenase-2: Divergence from Plant Orthologs and Its Relationship to Hydrogen Tunneling Activation …
A Ohler, PE Taylor, JA Bledsoe, AT Iavarone… - ACS …, 2024 - ACS Publications
The oxidation of polyunsaturated fatty acids by lipoxygenases (LOXs) is initiated by a C–H
cleavage step in which the hydrogen atom is transferred quantum mechanically (ie, via …
cleavage step in which the hydrogen atom is transferred quantum mechanically (ie, via …
Biochemical and hydrogen-deuterium exchange studies of the single nucleotide polymorphism Y649C in human platelet 12-lipoxygenase linked to a bleeding …
M Tran, RL Signorelli, A Yamaguchi, E Chen… - Archives of biochemistry …, 2023 - Elsevier
Abstract Human platelet 12-lipoxygenase (h12-LOX) is responsible for the formation of
oxylipin products that play an important role in platelet aggregation. Single nucleotide …
oxylipin products that play an important role in platelet aggregation. Single nucleotide …
Thermodynamic and biophysical study of fatty acid effector binding to soybean lipoxygenase: implications for allostery driven by helix α2 dynamics
DE Roberts, AM Benton, C Fabian‐Bayola… - FEBS …, 2022 - Wiley Online Library
Previous comparative kinetic isotope effects have inferred an allosteric site for fatty acids
and their derivatives that modulates substrate selectivity in 15‐lipoxygenases. Hydrogen …
and their derivatives that modulates substrate selectivity in 15‐lipoxygenases. Hydrogen …