Optical microscopes are an important imaging tool that have effectively advanced the
development of modern biomedicine. In recent years, super-resolution microscopy (SRM) …

The observation of molecular diffusion at different spatial scales, and in particular below the
optical diffraction limit (< 200 nm), can reveal details of the subcellular topology and its …

As stimulated emission depletion (STED) microscopy can provide structural details of cells
with an optical resolution beyond the diffraction limit, it has become an indispensable tool in …

The challenge of increasing the spatial resolution of an optical microscope beyond the
diffraction limit can be reduced to a spectroscopy task by proper manipulation of the …

We present an optical 3-D ranging camera for automotive applications that is able to provide
a centimeter depth resolution over a 40°× 20° field of view up to 45 m with just 1.5 W of …

This article deals with the developments of optical microscopy towards nanoscopy. Basic
concepts of the methods implemented to obtain spatial super-resolution are described …

The well-known saying of “Seeing is believing” became even more apt in biology when
stimulated emission depletion (STED) nanoscopy was introduced in 1994 by the Nobel …

Although deconvolution can improve the quality of any type of microscope, the high
computational time required has so far limited its massive spreading. Here we demonstrate …

Time-resolved fluorescence offers many advantages over normal steady-state detection and
becomes increasingly important in bioimaging. However, only very few fluorophores with …

Stimulated emission depletion microscopy (STED) is one of the pivotal super-resolution
techniques. It overcomes the spatial resolution limit imposed by the diffraction by using an …