Microbiological applications of high-resolution melting analysis

SYC Tong, PM Giffard - Journal of clinical microbiology, 2012 - Am Soc Microbiol
High-resolution melting (HRM) analysis uses real-time PCR instrumentation to interrogate
DNA sequence variation and is a low-cost, single-step, closed-tube method. Here we …

[HTML][HTML] Malaria diagnostics in clinical trials

SC Murphy, JP Shott, S Parikh, P Etter… - The American journal …, 2013 - ncbi.nlm.nih.gov
Malaria diagnostics are widely used in epidemiologic studies to investigate natural history of
disease and in drug and vaccine clinical trials to exclude participants or evaluate efficacy …

Modeling malaria genomics reveals transmission decline and rebound in Senegal

RF Daniels, SF Schaffner, EA Wenger… - Proceedings of the …, 2015 - National Acad Sciences
To study the effects of malaria-control interventions on parasite population genomics, we
examined a set of 1,007 samples of the malaria parasite Plasmodium falciparum collected in …

A Biotinylated cpFIT-PNA Platform for the Facile Detection of Drug Resistance to Artemisinin in Plasmodium falciparum

O Tepper, DH Appella, H Zheng, R Dzikowski… - ACS …, 2024 - ACS Publications
The evolution of drug resistance to many antimalarial drugs in the lethal strain of malaria
(Plasmodium falciparum) has been a great concern over the past 50 years. Among these …

The cytoplasmic prolyl-tRNA synthetase of the malaria parasite is a dual-stage target of febrifugine and its analogs

JD Herman, LR Pepper, JF Cortese, G Estiu… - Science translational …, 2015 - science.org
The emergence of drug resistance is a major limitation of current antimalarials. The
discovery of new druggable targets and pathways including those that are critical for multiple …

High throughput resistance profiling of Plasmodium falciparum infections based on custom dual indexing and Illumina next generation sequencing-technology

S Nag, MD Dalgaard, PE Kofoed, J Ursing, M Crespo… - Scientific reports, 2017 - nature.com
Genetic polymorphisms in P. falciparum can be used to indicate the parasite's susceptibility
to antimalarial drugs as well as its geographical origin. Both of these factors are key to …

Development of a Single Nucleotide Polymorphism Barcode to Genotype Plasmodium vivax Infections

ML Baniecki, AL Faust, SF Schaffner… - PLoS neglected …, 2015 - journals.plos.org
Plasmodium vivax, one of the five species of Plasmodium parasites that cause human
malaria, is responsible for 25–40% of malaria cases worldwide. Malaria global elimination …

Clonal Outbreak of Plasmodium falciparum Infection in Eastern Panama

N Obaldia III, NK Baro, JE Calzada… - The Journal of …, 2015 - academic.oup.com
Identifying the source of resurgent parasites is paramount to a strategic, successful
intervention for malaria elimination. Although the malaria incidence in Panama is low, a …

Rebound of multiple infections and prevalence of anti-malarial resistance associated markers following malaria upsurges in Dielmo village, Senegal, West Africa

AN Wotodjo, MA Oboh, S Doucoure, N Diagne… - Malaria journal, 2023 - Springer
Background Thanks to the scale up of malaria control interventions, the malaria burden in
Senegal has decreased substantially to the point that the National Malaria Control …

In vitro selection predicts malaria parasite resistance to dihydroorotate dehydrogenase inhibitors in a mouse infection model

REK Mandt, MJ Lafuente-Monasterio… - Science translational …, 2019 - science.org
Resistance has developed in Plasmodium malaria parasites to every antimalarial drug in
clinical use, prompting the need to characterize the pathways mediating resistance. Here …