[HTML][HTML] Applications of fluorescence spectroscopy in protein conformational changes and intermolecular contacts

FH dos Santos Rodrigues, GG Delgado, TS da Costa… - BBA advances, 2023 - Elsevier
Emission fluorescence is one of the most versatile and powerful biophysical techniques
used in several scientific subjects. It is extensively applied in the studies of proteins, their …

Fluorescent protein-based sensors for detecting essential metal ions across the tree of life

GC Jensen, MK Janis, HN Nguyen, OW David… - ACS …, 2024 - ACS Publications
Genetically encoded fluorescent metal ion sensors are powerful tools for elucidating metal
dynamics in living systems. Over the last 25 years since the first examples of genetically …

Genetically encoded fluorescent sensors for metals in biology

AP Torres-Ocampo, AE Palmer - Current opinion in chemical biology, 2023 - Elsevier
Metal ions intersect a wide range of biological processes. Some metal ions are essential
and hence absolutely required for the growth and health of an organism, others are toxic …

Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals

AMM Gest, AZ Sahan, Y Zhong, W Lin, S Mehta… - Chemical …, 2024 - ACS Publications
Cellular function is controlled through intricate networks of signals, which lead to the myriad
pathways governing cell fate. Fluorescent biosensors have enabled the study of these …

Enhanced small green fluorescent proteins as a multisensing platform for biosensor development

GT Liang, C Lai, Z Yue, H Zhang, D Li… - … in Bioengineering and …, 2022 - frontiersin.org
Engineered light, oxygen, and voltage (LOV)-based proteins are able to fluoresce without
oxygen requirement due to the autocatalytic incorporation of exogenous flavin as a …

[HTML][HTML] Metal-induced fluorescence quenching of photoconvertible fluorescent protein DendFP

IJ Kim, Y Xu, KH Nam - Molecules, 2022 - mdpi.com
Sensitive and accurate detection of specific metal ions is important for sensor development
and can advance analytical science and support environmental and human medical …

Mutant Flavin-Based Fluorescent Protein Sensors for Detecting Intracellular Zinc and Copper in Escherichia coli

W Zou, HN Nguyen, ML Zastrow - ACS sensors, 2022 - ACS Publications
Flavin-based fluorescent proteins (FbFPs) are a class of fluorescent reporters that undergo
oxygen-independent fluorophore incorporation, which is an important advantage over green …

Fluorescent protein-based metal biosensors

KH Nam - Chemosensors, 2023 - mdpi.com
Fluorescent proteins (FPs) are optical probes that are used to track the functions of
genetically encoded target molecules in molecular and cellular biology. FPs have intrinsic …

Development of an aptasensor for dibutyl phthalate detection and the elucidation of assay inhibition factors

H Song, HJ Lim, A Son - RSC advances, 2024 - pubs.rsc.org
We developed a fluorescence aptasensor (hereafter 'SG-aptasensor') using SYBR Green I, a
newly truncated 20-mer aptamer, and probe DNA to detect dibutyl phthalate (DBP). The …

Zinc-Induced Fluorescence Turn-On in Native and Mutant Phycoerythrobilin-Binding Orange Fluorescent Proteins

GC Jensen, MK Janis, J Jara, N Abbasi… - Biochemistry, 2023 - ACS Publications
Cyanobacteriochrome (CBCR)-derived fluorescent proteins are a class of reporters that can
bind bilin cofactors and fluoresce across the ultraviolet to the near-infrared spectrum …