Droplet-based digital PCR (ddPCR) and its applications

Y Hou, S Chen, Y Zheng, X Zheng, JM Lin - TrAC Trends in Analytical …, 2023 - Elsevier
As a new generation absolute quantitive PCR technique, droplet-based digital PCR
(ddPCR) provides the benefits of high sensitivity, precision, and reproducibility. A low-cost …

Concise whole blood transcriptional signatures for incipient tuberculosis: a systematic review and patient-level pooled meta-analysis

RK Gupta, CT Turner, C Venturini, H Esmail… - The Lancet …, 2020 - thelancet.com
Background Multiple blood transcriptional signatures have been proposed for identification
of active and incipient tuberculosis. We aimed to compare the performance of systematically …

Blood transcriptional biomarkers for active pulmonary tuberculosis in a high-burden setting: a prospective, observational, diagnostic accuracy study

CT Turner, RK Gupta, E Tsaliki, JK Roe… - The Lancet …, 2020 - thelancet.com
Background Blood transcriptional signatures are candidates for non-sputum triage or
confirmatory tests of tuberculosis. Prospective head-to-head comparisons of their diagnostic …

Diagnosis of multisystem inflammatory syndrome in children by a whole-blood transcriptional signature

HR Jackson, L Miglietta… - Journal of the …, 2023 - academic.oup.com
Background To identify a diagnostic blood transcriptomic signature that distinguishes
multisystem inflammatory syndrome in children (MIS-C) from Kawasaki disease (KD) …

Blood transcriptomic signatures for symptomatic tuberculosis in an African multicohort study

VM Muwanga, SC Mendelsohn… - European …, 2024 - publications.ersnet.org
Background Multiple host blood transcriptional signatures have been developed as non-
sputum triage tests for tuberculosis (TB). We aimed to compare the diagnostic performance …

Transcriptional profiling and deriving a seven-gene signature that discriminates active and latent tuberculosis: an integrative bioinformatics approach

S Natarajan, M Ranganathan, LE Hanna, S Tripathy - Genes, 2022 - mdpi.com
Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (M. tb.).
Our integrative analysis aims to identify the transcriptional profiling and gene expression …

[HTML][HTML] Gene signature discovery and systematic validation across diverse clinical cohorts for TB prognosis and response to treatment

R Vargas, L Abbott, D Bower, N Frahm… - PLOS Computational …, 2023 - journals.plos.org
While blood gene signatures have shown promise in tuberculosis (TB) diagnosis and
treatment monitoring, most signatures derived from a single cohort may be insufficient to …

Specific human gene expression in response to infection is an effective marker for diagnosis of latent and active tuberculosis

R Nakiboneka, N Walbaum, E Musisi, M Nevels… - Scientific Reports, 2024 - nature.com
RNA sequencing and microarray analysis revealed transcriptional markers expressed in
whole blood can differentiate active pulmonary TB (ATB) from other respiratory diseases …

Identification of hub genes for adult patients with sepsis via RNA sequencing

Q Zhang, Y Hu, P Wei, L Shi, L Shi, J Li, Y Zhao… - Scientific Reports, 2022 - nature.com
To screen out potential prognostic hub genes for adult patients with sepsis via RNA
sequencing and construction of a microRNA–mRNA–PPI network and investigate the …

Novel RNA biomarkers improve discrimination of children with tuberculosis disease from those with non-TB pneumonia after in vitro stimulation

O Vito, S Psarras, A Syggelou, VJ Wright… - Frontiers in …, 2024 - frontiersin.org
The diagnosis of pediatric tuberculosis (TB) poses a challenge for clinical teams worldwide.
TB-mediated changes in the expression of host genes in the peripheral blood can serve as …