The historical reliance of biological research on the use of animal models has sometimes
made it challenging to address questions that are specific to the understanding of human …

Biomedical research is undergoing a paradigm shift towards approaches centred on human
disease models owing to the notoriously high failure rates of the current drug development …

Adult stem cell–based organoid technology is a versatile tool for the generation and long-
term maintenance of near-native 3D epithelial tissues in vitro. The generation of cancer …

Simple Summary Cell cultures can be carried out in three dimensions (3D). Organoids and
spheroids are different 3D cell culture models that can be cultured with different techniques …

Ovarian cancer (OC) is a heterogeneous disease usually diagnosed at a late stage.
Experimental in vitro models that faithfully capture the hallmarks and tumor heterogeneity of …

Tissue and organ biology are very challenging to study in mammals, and progress can be
hindered, particularly in humans, by sample accessibility and ethical concerns. However …

The recent advances in in vitro 3D culture technologies, such as organoids, have opened
new avenues for the development of novel, more physiological human cancer models. Such …

In vitro cell cultures are crucial research tools for modeling human development and
diseases. Although the conventional monolayer cell cultures have been widely used in the …

Organoids are in vitro–cultured three-dimensional structures that recapitulate key aspects of
in vivo organs. They can be established from pluripotent stem cells and from adult stem …

The WNT signal transduction cascade is a main regulator of development throughout the
animal kingdom. Wnts are also key drivers of most types of tissue stem cells in adult …