Molecular motors are diverse enzymes that transduce chemical energy into mechanical
work and, in doing so, perform critical cellular functions such as DNA replication and …

Force spectroscopy has become an indispensable tool to unravel the structural and
mechanochemical properties of biomolecules. Here we extend the force spectroscopy …

Sequence-specific DNA-binding proteins can maintain and regulate cellular functions by
accurately and quickly binding to target sequences among large amounts of nontarget DNA …

The helical structure of double-stranded DNA is destabilized by increasing temperature.
Above a critical temperature (the melting temperature), the two strands in duplex DNA …

Mitochondria organize their genome in protein–DNA complexes called nucleoids. The
mitochondrial transcription factor A (TFAM), a protein that regulates mitochondrial …

We describe a tethered multifluorophore motion assay based on DNA origami for revealing
bimolecular reaction kinetics on the single-molecule level. Molecular binding partners may …

The inflexibility of double-stranded DNA with respect to bending and twisting is well
established in vitro. Understanding apparent DNA physical properties in vivo is a greater …

We present a new method for inferring hidden Markov models from noisy time sequences
without the necessity of assuming a model architecture, thus allowing for the detection of …

Tethered-particle motion experiments do not require expensive or technically complex
hardware, and increasing numbers of researchers are adopting this methodology to …

Many DNA regulatory factors require communication between distantly separated DNA sites
for their activity. The type IIF restriction enzyme SfiI is often used as a model system of site …