A guide to studying protein aggregation

JAJ Housmans, G Wu, J Schymkowitz… - The FEBS …, 2023‏ - Wiley Online Library
Disrupted protein folding or decreased protein stability can lead to the accumulation of
(partially) un‐or misfolded proteins, which ultimately cause the formation of protein …

Fluorescence-based protein stability monitoring—a review

N Gooran, K Kopra - International Journal of Molecular Sciences, 2024‏ - mdpi.com
Proteins are large biomolecules with a specific structure that is composed of one or more
long amino acid chains. Correct protein structures are directly linked to their correct function …

Measuring protein structural changes on a proteome-wide scale using limited proteolysis-coupled mass spectrometry

S Schopper, A Kahraman, P Leuenberger, Y Feng… - Nature protocols, 2017‏ - nature.com
Protein structural changes induced by external perturbations or internal cues can profoundly
influence protein activity and thus modulate cellular physiology. A number of biophysical …

Surface chemistry-mediated modulation of adsorbed albumin folding state specifies nanocarrier clearance by distinct macrophage subsets

MP Vincent, S Bobbala, NB Karabin, M Frey… - Nature …, 2021‏ - nature.com
Controlling nanocarrier interactions with the immune system requires a thorough
understanding of the surface properties that modulate protein adsorption in biological fluids …

[HTML][HTML] Refolding in the modern biopharmaceutical industry

L Buscajoni, MC Martinetz, M Berkemeyer… - Biotechnology …, 2022‏ - Elsevier
Inclusion bodies (IBs) often emerge upon overexpression of recombinant proteins in E. coli.
From IBs, refolding is necessary to generate the native protein that can be further purified to …

Scalable production of genetically engineered nanofibrous macroscopic materials via filtration

NM Dorval Courchesne, A Duraj-Thatte… - ACS Biomaterials …, 2017‏ - ACS Publications
As interest in using proteins to assemble functional, biocompatible, and environmentally
friendly materials is growing, develo** scalable protocols for producing recombinant …

Virus-like Particles Armored by an Endoskeleton

Z Wu, JL Bayón, TB Kouznetsova, T Ouchi… - Nano Letters, 2024‏ - ACS Publications
Many virus-like particles (VLPs) have good chemical, thermal, and mechanical stabilities
compared to those of other biologics. However, their stability needs to be improved for the …

Changing relations between proteins and osmolytes: a choice of nature

A Rani, P Venkatesu - Physical Chemistry Chemical Physics, 2018‏ - pubs.rsc.org
Many studies aimed at advancing our understanding of the mechanisms of protein
stabilization that support the survival of organisms under harsh environmental conditions are …

Rapid Coaggregation of Proteins Without Sequence Similarity: Possible Role of Conformational Complementarity

KP Prajapati, M Ansari, S Mittal, N Mishra, A Bhatia… - Biochemistry, 2024‏ - ACS Publications
Despite extensive research on the sequence-determined self-assembly of both pathogenic
and nonpathogenic proteins, the question of how the sequence identity would influence the …

Temperature-Induced Luminescence Intensity Fluctuation of Protein-Protected Copper Nanoclusters: Role of Scaffold Conformation vs Nonradiative Transition

A Sebastian, KP, Aarya, S Sen Mojumdar - ACS omega, 2024‏ - ACS Publications
Protein-scaffolded atomically precise metal nanoclusters (NCs) have emerged as a
promising class of biofriendly nanoprobes at the forefront of modern research, particularly in …