Splicing regulation through biomolecular condensates and membraneless organelles

J Giudice, H Jiang - Nature Reviews Molecular Cell Biology, 2024 - nature.com
Biomolecular condensates, sometimes also known as membraneless organelles (MLOs),
can form through weak multivalent intermolecular interactions of proteins and nucleic acids …

Measuring age-dependent viscoelasticity of organelles, cells and organisms with time-shared optical tweezer microrheology

F Català-Castro, S Ortiz-Vásquez… - Nature …, 2025 - nature.com
Quantifying the mechanical response of the biological milieu (such as the cell's interior) and
complex fluids (such as biomolecular condensates) would enable a better understanding of …

Conformational ensembles of the human intrinsically disordered proteome: Bridging chain compaction with function and sequence conservation

G Tesei, AI Trolle, N Jonsson, J Betz, F Pesce… - bioRxiv, 2023 - biorxiv.org
Intrinsically disordered proteins and regions (collectively IDRs) are pervasive across
proteomes in all kingdoms of life, help shape biological functions, and are involved in …

A pH-Dependent Coarse-Grained Model for Disordered Proteins: Histidine Interactions Modulate Conformational Ensembles

R Calinsky, Y Levy - The Journal of Physical Chemistry Letters, 2024 - ACS Publications
Histidine (His) presents a unique challenge for modeling disordered protein conformations,
as it is versatile and occurs in both the neutral (His0) and positively charged (His+) states …

Accurate long-read transcript discovery and quantification at single-cell, pseudo-bulk and bulk resolution with Isosceles

M Kabza, A Ritter, A Byrne, K Sereti, D Le… - Nature …, 2024 - nature.com
Accurate detection and quantification of mRNA isoforms from nanopore long-read
sequencing remains challenged by technical noise, particularly in single cells. To address …

Core-shell model of the clusters of CPEB4 isoforms preceding liquid-liquid phase separation

M Oranges, C Jash, G Golani, M Seal, SR Cohen… - Biophysical …, 2024 - cell.com
Protein solutions can undergo liquid-liquid phase separation (LLPS), where a dispersed
phase with a low protein concentration coexists with coacervates with a high protein …

Cytoplasmic Regulation of the Poly (A) Tail Length as a Potential Therapeutic Target

M Fernandez, R Mendez - RNA, 2025 - rnajournal.cshlp.org
Virtually all mRNAs acquire a poly (A) tail co-transcriptionally, but its length is dynamically
regulated in the cytoplasm in a transcript-specific manner. The length of the poly (A) tail …

[HTML][HTML] Metastable condensates suppress conversion to amyloid fibrils

T Das, F Zaidi, M Farag, KM Ruff, J Messing, JP Taylor… - bioRxiv, 2024 - ncbi.nlm.nih.gov
Stress granules form via co-condensation of RNA binding proteins with prion-like low
complexity domains (PLCDs) and RNA molecules released by stress-induced polysomal …

Microexon in action: How tiny fragments in a protein tune function, drive disease

J Huang - Molecular Cell, 2025 - cell.com
Intrinsically disordered regions (IDRs) of proteins can regulate function through phase
separation. In a recent article in Nature, Garcia-Cabau et al. reveal that including or …

Measuring age-dependent viscoelastic properties of organelles, cells and organisms via Time-Shared Optical Tweezer Microrheology

F Català-Castro, S Ortiz-Vásquez… - bioRxiv, 2023 - biorxiv.org
Recording the mechanical response of biological samples, the cell's interior and complex
fluids in general, would enable deeper understanding of cellular differentiation, ageing and …