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Beta-barrel scaffold of fluorescent proteins: folding, stability and role in chromophore formation
This review focuses on the current view of the interaction between the β-barrel scaffold of
fluorescent proteins and their unique chromophore located in the internal helix. The …
fluorescent proteins and their unique chromophore located in the internal helix. The …
Advances in single quantum dot-based nanosensors
J Hu, Z Wang, C Li, C Zhang - Chemical Communications, 2017 - pubs.rsc.org
Single-molecule detection provides a simple and ultrasensitive platform to quantify target
molecules by simply counting the individual fluorescence signals. Quantum dots (QDs) are …
molecules by simply counting the individual fluorescence signals. Quantum dots (QDs) are …
Direct observation of the interconversion of normal and toxic forms of α-synuclein
N Cremades, SIA Cohen, E Deas, AY Abramov… - Cell, 2012 - cell.com
Here, we use single-molecule techniques to study the aggregation of α-synuclein, the
protein whose misfolding and deposition is associated with Parkinson's disease. We identify …
protein whose misfolding and deposition is associated with Parkinson's disease. We identify …
Kinetic model of the aggregation of alpha-synuclein provides insights into prion-like spreading
The protein alpha-synuclein (αS) self-assembles into small oligomeric species and
subsequently into amyloid fibrils that accumulate and proliferate during the development of …
subsequently into amyloid fibrils that accumulate and proliferate during the development of …
A mechanistic model of tau amyloid aggregation based on direct observation of oligomers
Protein aggregation plays a key role in neurodegenerative disease, giving rise to small
oligomers that may become cytotoxic to cells. The fundamental microscopic reactions taking …
oligomers that may become cytotoxic to cells. The fundamental microscopic reactions taking …
Accurate single-molecule FRET studies using multiparameter fluorescence detection
In the recent decade, single-molecule (sm) spectroscopy has come of age and is providing
important insight into how biological molecules function. So far our view of protein function is …
important insight into how biological molecules function. So far our view of protein function is …
Ubiquitin chain conformation regulates recognition and activity of interacting proteins
Mechanisms of protein recognition have been extensively studied for single-domain
proteins, but are less well characterized for dynamic multidomain systems. Ubiquitin chains …
proteins, but are less well characterized for dynamic multidomain systems. Ubiquitin chains …
Hsp70 inhibits the nucleation and elongation of tau and sequesters tau aggregates with high affinity
As a key player of the protein quality control network of the cell, the molecular chaperone
Hsp70 inhibits the aggregation of the amyloid protein tau. To date, the mechanism of this …
Hsp70 inhibits the aggregation of the amyloid protein tau. To date, the mechanism of this …
Direct characterization of amyloidogenic oligomers by single-molecule fluorescence
A key issue in understanding the pathogenic conditions associated with the aberrant
aggregation of misfolded proteins is the identification and characterization of species formed …
aggregation of misfolded proteins is the identification and characterization of species formed …
Single quantum dot-based nanosensor for multiple DNA detection
C Zhang, J Hu - Analytical chemistry, 2010 - ACS Publications
Owing to their unique optical properties, quantum dots (QDs) with different colors have been
applied for simultaneous detection of multiple analytes. However, the use of single QD for …
applied for simultaneous detection of multiple analytes. However, the use of single QD for …