Beyond the RNA-dependent function of LncRNA genes

T Ali, P Grote - Elife, 2020 - elifesciences.org
While long non-coding RNA (lncRNA) genes have attracted a lot of attention in the last
decade, the focus regarding their mechanisms of action has been primarily on the RNA …

3′ UTR heterogeneity and cancer progression

JJ Chan, H Tabatabaeian, Y Tay - Trends in Cell Biology, 2023 - cell.com
The majority of human mRNAs generate alternative 3′ untranslated regions (UTRs)
through various processes, including RNA modifications such as RNA editing, m 6 A …

Exon architecture controls mRNA m6A suppression and gene expression

PC He, J Wei, X Dou, BT Harada, Z Zhang, R Ge, C Liu… - Science, 2023 - science.org
N 6-methyladenosine (m6A) is the most abundant messenger RNA (mRNA) modification
and plays crucial roles in diverse physiological processes. Using a massively parallel assay …

Predicting RNA-seq coverage from DNA sequence as a unifying model of gene regulation

J Linder, D Srivastava, H Yuan, V Agarwal, DR Kelley - Nature Genetics, 2025 - nature.com
Sequence-based machine-learning models trained on genomics data improve genetic
variant interpretation by providing functional predictions describing their impact on the cis …

High-throughput total RNA sequencing in single cells using VASA-seq

F Salmen, J De Jonghe, TS Kaminski, A Alemany… - Nature …, 2022 - nature.com
Most methods for single-cell transcriptome sequencing amplify the termini of polyadenylated
transcripts, capturing only a small fraction of the total cellular transcriptome. This precludes …

TDP-43 condensation properties specify its RNA-binding and regulatory repertoire

M Hallegger, AM Chakrabarti, FCY Lee, BL Lee… - Cell, 2021 - cell.com
Mutations causing amyotrophic lateral sclerosis (ALS) often affect the condensation
properties of RNA-binding proteins (RBPs). However, the role of RBP condensation in the …

Long 3′ UTRs predispose neurons to inflammation by promoting immunostimulatory double-stranded RNA formation

TJ Dorrity, H Shin, KA Wiegand, J Aruda… - Science …, 2023 - science.org
Loss of RNA homeostasis underlies numerous neurodegenerative and neuroinflammatory
diseases. However, the molecular mechanisms that trigger neuroinflammation are poorly …

Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue

SA Hardwick, W Hu, A Joglekar, L Fan, PG Collier… - Nature …, 2022 - nature.com
Single-nuclei RNA sequencing characterizes cell types at the gene level. However,
compared to single-cell approaches, many single-nuclei cDNAs are purely intronic, lack …

A spatially resolved brain region-and cell type-specific isoform atlas of the postnatal mouse brain

A Joglekar, A Prjibelski, A Mahfouz, P Collier… - Nature …, 2021 - nature.com
Splicing varies across brain regions, but the single-cell resolution of regional variation is
unclear. We present a single-cell investigation of differential isoform expression (DIE) …

SQANTI3: curation of long-read transcriptomes for accurate identification of known and novel isoforms

FJ Pardo-Palacios, A Arzalluz-Luque, L Kondratova… - Nature …, 2024 - nature.com
SQANTI3 is a tool designed for the quality control, curation and annotation of long-read
transcript models obtained with third-generation sequencing technologies. Leveraging its …