Macromolecular modeling and design in Rosetta: recent methods and frameworks

JK Leman, BD Weitzner, SM Lewis, J Adolf-Bryfogle… - Nature …, 2020 - nature.com
The Rosetta software for macromolecular modeling, docking and design is extensively used
in laboratories worldwide. During two decades of development by a community of …

Recent advances in de novo protein design: Principles, methods, and applications

X Pan, T Kortemme - Journal of Biological Chemistry, 2021 - ASBMB
The computational de novo protein design is increasingly applied to address a number of
key challenges in biomedicine and biological engineering. Successes in expanding …

Cell-free chemoenzymatic starch synthesis from carbon dioxide

T Cai, H Sun, J Qiao, L Zhu, F Zhang, J Zhang, Z Tang… - Science, 2021 - science.org
Starches, a storage form of carbohydrates, are a major source of calories in the human diet
and a primary feedstock for bioindustry. We report a chemical-biochemical hybrid pathway …

Coarse-grained protein models and their applications

S Kmiecik, D Gront, M Kolinski, L Wieteska… - Chemical …, 2016 - ACS Publications
The traditional computational modeling of protein structure, dynamics, and interactions
remains difficult for many protein systems. It is mostly due to the size of protein …

Easy and accurate protein structure prediction using ColabFold

G Kim, S Lee, E Levy Karin, H Kim, Y Moriwaki… - Nature …, 2024 - nature.com
Since its public release in 2021, AlphaFold2 (AF2) has made investigating biological
questions, by using predicted protein structures of single monomers or full complexes, a …

Protein sequence design with a learned potential

N Anand, R Eguchi, II Mathews, CP Perez… - Nature …, 2022 - nature.com
The task of protein sequence design is central to nearly all rational protein engineering
problems, and enormous effort has gone into the development of energy functions to guide …

Advances in molecular dynamics simulations and enhanced sampling methods for the study of protein systems

R Lazim, D Suh, S Choi - International journal of molecular sciences, 2020 - mdpi.com
Molecular dynamics (MD) simulation is a rigorous theoretical tool that when used efficiently
could provide reliable answers to questions pertaining to the structure-function relationship …

[HTML][HTML] A smoothed backbone-dependent rotamer library for proteins derived from adaptive kernel density estimates and regressions

MV Shapovalov, RL Dunbrack - Structure, 2011 - cell.com
Rotamer libraries are used in protein structure determination, prediction, and design. The
backbone-dependent rotamer library consists of rotamer frequencies, mean dihedral angles …

RosettaScripts: a scripting language interface to the Rosetta macromolecular modeling suite

SJ Fleishman, A Leaver-Fay, JE Corn, EM Strauch… - PloS one, 2011 - journals.plos.org
Macromolecular modeling and design are increasingly useful in basic research,
biotechnology, and teaching. However, the absence of a user-friendly modeling framework …

Role of conformational sampling in computing mutation‐induced changes in protein structure and stability

EH Kellogg, A Leaver‐Fay… - … : Structure, Function, and …, 2011 - Wiley Online Library
The prediction of changes in protein stability and structure resulting from single amino acid
substitutions is both a fundamental test of macromolecular modeling methodology and an …