3D variability analysis: Resolving continuous flexibility and discrete heterogeneity from single particle cryo-EM
Single particle cryo-EM excels in determining static structures of protein molecules, but
existing 3D reconstruction methods have been ineffective in modelling flexible proteins. We …
existing 3D reconstruction methods have been ineffective in modelling flexible proteins. We …
The diversity of Shine-Dalgarno sequences sheds light on the evolution of translation initiation
ABSTRACT Shine-Dalgarno (SD) sequences, the core element of prokaryotic ribosome-
binding sites, facilitate mRNA translation by base-pair interaction with the anti-SD (aSD) …
binding sites, facilitate mRNA translation by base-pair interaction with the anti-SD (aSD) …
A 'Build and Retrieve'methodology to simultaneously solve cryo-EM structures of membrane proteins
Single-particle cryo-electron microscopy (cryo-EM) has become a powerful technique in the
field of structural biology. However, the inability to reliably produce pure, homogeneous …
field of structural biology. However, the inability to reliably produce pure, homogeneous …
Ribosome‐targeting antibacterial agents: Advances, challenges, and opportunities
L Zhang, J He, L Bai, S Ruan, T Yang… - Medicinal Research …, 2021 - Wiley Online Library
Ribosomes, which synthesize proteins, are critical organelles for the survival and growth of
bacteria. About 60% of approved antibiotics discovered so far combat pathogenic bacteria …
bacteria. About 60% of approved antibiotics discovered so far combat pathogenic bacteria …
Cryoelectron microscopy structures of AdeB illuminate mechanisms of simultaneous binding and exporting of substrates
Acinetobacter baumannii is a Gram-negative pathogen that has emerged as one of the most
highly antibiotic-resistant bacteria worldwide. Multidrug efflux within these highly drug …
highly antibiotic-resistant bacteria worldwide. Multidrug efflux within these highly drug …
Cryo-EM determination of eravacycline-bound structures of the ribosome and the multidrug efflux pump AdeJ of Acinetobacter baumannii
Antibiotic-resistant strains of the Gram-negative pathogen Acinetobacter baumannii have
emerged as a significant global health threat. One successful therapeutic option to treat …
emerged as a significant global health threat. One successful therapeutic option to treat …
Streptothricin F is a bactericidal antibiotic effective against highly drug-resistant gram-negative bacteria that interacts with the 30S subunit of the 70S ribosome
The streptothricin natural product mixture (also known as nourseothricin) was discovered in
the early 1940s, generating intense initial interest because of excellent gram-negative …
the early 1940s, generating intense initial interest because of excellent gram-negative …
An analysis of the novel fluorocycline TP-6076 bound to both the ribosome and multidrug efflux pump AdeJ from Acinetobacter baumannii
Antibiotic resistance among bacterial pathogens continues to pose a serious global health
threat. Multidrug-resistant (MDR) strains of the Gram-negative organism Acinetobacter …
threat. Multidrug-resistant (MDR) strains of the Gram-negative organism Acinetobacter …
Ratchet, swivel, tilt and roll: a complete description of subunit rotation in the ribosome
Protein synthesis by the ribosome requires large-scale rearrangements of the 'small'subunit
(SSU;∼ 1 MDa), including inter-and intra-subunit rotational motions. However, with nearly …
(SSU;∼ 1 MDa), including inter-and intra-subunit rotational motions. However, with nearly …
Structural heterogeneities of the ribosome: new frontiers and opportunities for cryo-EM
The extent of ribosomal heterogeneity has caught increasing interest over the past few
years, as recent studies have highlighted the presence of structural variations of the …
years, as recent studies have highlighted the presence of structural variations of the …