Confining enzymes in porous organic frameworks: from synthetic strategy and characterization to healthcare applications

S Huang, G Chen, G Ouyang - Chemical Society Reviews, 2022 - pubs.rsc.org
Enzymes are a class of natural catalysts with high efficiency, specificity, and selectivity
unmatched by their synthetic counterparts and dictate a myriad of reactions that constitute …

[HTML][HTML] Oriented immobilization of antibodies onto sensing platforms-A critical review

S Gao, JM Guisán, J Rocha-Martin - Analytica chimica acta, 2022 - Elsevier
The immunosensor has been proven a versatile tool to detect various analytes, such as food
contaminants, pathogenic bacteria, antibiotics and biomarkers related to cancer. To fabricate …

Industrial applications of enzymes: Recent advances, techniques, and outlooks

J Chapman, AE Ismail, CZ Dinu - Catalysts, 2018 - mdpi.com
Enzymes as industrial biocatalysts offer numerous advantages over traditional chemical
processes with respect to sustainability and process efficiency. Enzyme catalysis has been …

[HTML][HTML] Enzyme immobilization and co-immobilization: main framework, advances and some applications

J Bié, B Sepodes, PCB Fernandes, MHL Ribeiro - Processes, 2022 - mdpi.com
Enzymes are outstanding (bio) catalysts, not solely on account of their ability to increase
reaction rates by up to several orders of magnitude but also for the high degree of substrate …

Integrating enzyme immobilization and protein engineering: An alternative path for the development of novel and improved industrial biocatalysts

C Bernal, K Rodriguez, R Martinez - Biotechnology Advances, 2018 - Elsevier
Enzyme immobilization often achieves reusable biocatalysts with improved operational
stability and solvent resistance. However, these modifications are generally associated with …

Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts

O Barbosa, C Ortiz, Á Berenguer-Murcia, R Torres… - Biotechnology …, 2015 - Elsevier
In this review, we detail the efforts performed to couple the purification and the
immobilization of industrial enzymes in a single step. The use of antibodies, the …

Immobilization of enzymes on porous silicas–benefits and challenges

M Hartmann, X Kostrov - Chemical Society Reviews, 2013 - pubs.rsc.org
Porous silica materials have extensively been used for the immobilization of enzymes
aiming at their use as biocatalysts or biosensors. This tutorial review will discuss the benefits …

Silica surface features and their role in the adsorption of biomolecules: computational modeling and experiments

A Rimola, D Costa, M Sodupe, JF Lambert… - Chemical …, 2013 - ACS Publications
There are many reasons to report on silica interacting with biomolecules. The most obvious
one is that on the Earth's crust, oxygen and silicon are the most abundant atomic species …

Streamlining design, engineering, and applications of enzymes for sustainable biocatalysis

RA Sheldon, D Brady - ACS Sustainable Chemistry & Engineering, 2021 - ACS Publications
In this Perspective we show how an expansion of the scope and impact of biocatalysis in
industrial organic synthesis is enabled by streamlining the underpinning biocatalyst and …

Combining a Genetically Engineered Oxidase with Hydrogen‐Bonded Organic Frameworks (HOFs) for Highly Efficient Biocomposites

P Wied, F Carraro, JM Bolivar… - Angewandte Chemie …, 2022 - Wiley Online Library
Enzymes incorporated into hydrogen‐bonded organic frameworks (HOFs) via bottom‐up
synthesis are promising biocomposites for applications in catalysis and sensing. Here, we …