Non-AUG translation initiation in mammals

DE Andreev, G Loughran, AD Fedorova… - Genome Biology, 2022 - Springer
Recent proteogenomic studies revealed extensive translation outside of annotated protein
coding regions, such as non-coding RNAs and untranslated regions of mRNAs. This non …

Surviving and adapting to stress: translational control and the integrated stress response

RC Wek, TG Anthony, KA Staschke - Antioxidants & Redox …, 2023 - liebertpub.com
Significance: Organisms adapt to changing environments by engaging cellular stress
response pathways that serve to restore proteostasis and enhance survival. A primary …

mRNA structure regulates protein expression through changes in functional half-life

DM Mauger, BJ Cabral, V Presnyak… - Proceedings of the …, 2019 - National Acad Sciences
Messenger RNAs (mRNAs) encode information in both their primary sequence and their
higher order structure. The independent contributions of factors like codon usage and …

CRISPR/Cas13 effectors have differing extents of off-target effects that limit their utility in eukaryotic cells

Y Ai, D Liang, JE Wilusz - Nucleic Acids Research, 2022 - academic.oup.com
CRISPR/Cas13 effectors have garnered increasing attention as easily customizable tools for
detecting and depleting RNAs of interest. Near perfect complementarity between a target …

Selective 40S footprinting reveals cap-tethered ribosome scanning in human cells

J Bohlen, K Fenzl, G Kramer, B Bukau, AA Teleman - Molecular cell, 2020 - cell.com
Translation regulation occurs largely during the initiation phase. Here, we develop selective
40S footprinting to visualize initiating 40S ribosomes on endogenous mRNAs in vivo. This …

Ribosome inhibition by C9ORF72-ALS/FTD-associated poly-PR and poly-GR proteins revealed by cryo-EM

AB Loveland, E Svidritskiy, D Susorov, S Lee… - Nature …, 2022 - nature.com
Toxic dipeptide-repeat (DPR) proteins are produced from expanded G4C2 repeats in the
C9ORF72 gene, the most common genetic cause of amyotrophic lateral sclerosis (ALS) and …

iRQC, a surveillance pathway for 40S ribosomal quality control during mRNA translation initiation

DM Garshott, H An, E Sundaramoorthy, M Leonard… - Cell reports, 2021 - cell.com
Post-translational modification of ribosomal proteins enables rapid and dynamic regulation
of protein biogenesis. Site-specific ubiquitylation of 40S ribosomal proteins uS10 and eS10 …

Nuclear release of eIF1 restricts start-codon selection during mitosis

J Ly, K **ang, KC Su, GB Sissoko, DP Bartel… - Nature, 2024 - nature.com
Regulated start-codon selection has the potential to reshape the proteome through the
differential production of upstream open reading frames, canonical proteins, and alternative …

Humans and other commonly used model organisms are resistant to cycloheximide-mediated biases in ribosome profiling experiments

P Sharma, J Wu, BS Nilges, SA Leidel - Nature Communications, 2021 - nature.com
Ribosome profiling measures genome-wide translation dynamics at sub-codon resolution.
Cycloheximide (CHX), a widely used translation inhibitor to arrest ribosomes in these …

Small open reading frames, how to find them and determine their function

PM Kute, O Soukarieh, H Tjeldnes, DA Trégouët… - Frontiers in …, 2022 - frontiersin.org
Advances in genomics and molecular biology have revealed an abundance of small open
reading frames (sORFs) across all types of transcripts. While these sORFs are often …