Prospects and challenges of environmental DNA (eDNA) monitoring in freshwater ponds

LR Harper, AS Buxton, HC Rees, K Bruce, R Brys… - Hydrobiologia, 2019 - Springer
Environmental DNA (eDNA) analysis is a rapid, non-invasive, cost-efficient biodiversity
monitoring tool with enormous potential to inform aquatic conservation and management …

Fundamental research questions in subterranean biology

S Mammola, IR Amorim, ME Bichuette… - Biological …, 2020 - Wiley Online Library
Five decades ago, a landmark paper in Science titled The Cave Environment heralded
caves as ideal natural experimental laboratories in which to develop and address general …

Finding answers in the dark: caves as models in ecology fifty years after Poulson and White

S Mammola - Ecography, 2019 - Wiley Online Library
The use of semi‐isolated habitats such as oceanic islands, lakes and mountain summits as
model systems has played a crucial role in the development of evolutionary and ecological …

[HTML][HTML] eDNA in subterranean ecosystems: Applications, technical aspects, and future prospects

M Saccò, MT Guzik, M van der Heyde, P Nevill… - Science of the Total …, 2022 - Elsevier
Monitoring of biota is pivotal for the assessment and conservation of ecosystems.
Environments worldwide are being continuously and increasingly exposed to multiple …

[HTML][HTML] Applications of environmental DNA (eDNA) to detect subterranean and aquatic invasive species: a critical review on the challenges and limitations of eDNA …

ST Rishan, RJ Kline, MS Rahman - Environmental Advances, 2023 - Elsevier
The world is struggling to solve a devastating biodiversity loss that not only affects the
extinction of treasured species and irreplaceable genetic variation, but also jeopardizes the …

Integrating citizen science and environmental DNA metabarcoding to study biodiversity of groundwater amphipods in Switzerland

M Couton, A Studer, S Hürlemann, N Locher… - Scientific Reports, 2023 - nature.com
Groundwater is the physically largest freshwater ecosystem, yet one of the least explored
habitats on earth, both because of accessing difficulties and the scarcity of the organisms …

Development and evaluation of fish eDNA metabarcoding assays facilitate the detection of cryptic seahorse taxa (family: Syngnathidae)

GM Nester, M De Brauwer, A Koziol… - Environmental …, 2020 - Wiley Online Library
Environmental DNA (eDNA) metabarcoding methods have demonstrated their potential as
noninvasive techniques for the monitoring and conservation of marine fishes, including rare …

Taxonomic and geographical representation of freshwater environmental DNA research in aquatic conservation

CC Belle, BC Stoeckle, J Geist - Aquatic Conservation: Marine …, 2019 - Wiley Online Library
Freshwater macro‐organismic environmental DNA (eDNA) is gaining increasing popularity
in detecting invasive species, assessing community assemblages, and in map** the …

Collecting eco‐evolutionary data in the dark: Impediments to subterranean research and how to overcome them

S Mammola, E Lunghi, H Bilandžija… - Ecology and …, 2021 - Wiley Online Library
Caves and other subterranean habitats fulfill the requirements of experimental model
systems to address general questions in ecology and evolution. Yet, the harsh working …

[HTML][HTML] Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample types

GS Sellers, C Di Muri, A Gómez… - Metabarcoding and …, 2018 - mbmg.pensoft.net
Efficient DNA extraction is fundamental to molecular studies. However, commercial kits are
expensive when a large number of samples need to be processed. Here we present a …