FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices

E Lerner, A Barth, J Hendrix, B Ambrose, V Birkedal… - Elife, 2021 - elifesciences.org
Single-molecule FRET (smFRET) has become a mainstream technique for studying
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …

Do-it-yourself guide: how to use the modern single-molecule toolkit

NG Walter, CY Huang, AJ Manzo, MA Sobhy - Nature methods, 2008 - nature.com
Single-molecule microscopy has evolved into the ultimate-sensitivity toolkit to study systems
from small molecules to living cells, with the prospect of revolutionizing the modern …

DNA catalysis: the chemical repertoire of DNAzymes

M Hollenstein - Molecules, 2015 - mdpi.com
Deoxyribozymes or DNAzymes are single-stranded catalytic DNA molecules that are
obtained by combinatorial in vitro selection methods. Initially conceived to function as gene …

[HTML][HTML] Single-molecule four-color FRET

J Lee, S Lee, K Ragunathan, C Joo, T Ha… - … (International ed. in …, 2010 - ncbi.nlm.nih.gov
Single-molecule Four-color FRET - PMC Back to Top Skip to main content NIH NLM Logo
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation Preview …

A versatile endoribonuclease mimic made of DNA: characteristics and applications of the 8–17 RNA‐cleaving DNAzyme

K Schlosser, Y Li - ChemBioChem, 2010 - Wiley Online Library
Enzymes play a crucial role in all living organisms by accelerating the rates of a myriad of
biochemical reactions that are necessary to sustain life. Although the vast majority of known …

Multi-parameter photon-by-photon hidden Markov modeling

PD Harris, A Narducci, C Gebhardt, T Cordes… - Nature …, 2022 - nature.com
Single molecule Förster resonance energy transfer (smFRET) is a unique biophysical
approach for studying conformational dynamics in biomacromolecules. Photon-by-photon …

Maximizing information content of single-molecule FRET experiments: multi-color FRET and FRET combined with force or torque

S Hohng, S Lee, J Lee, MH Jo - Chemical Society Reviews, 2014 - pubs.rsc.org
Since its first demonstration about twenty years ago, single-molecule fluorescence
resonance energy transfer (FRET) has undergone remarkable technical advances. In this …

Insights into DNA catalysis from structural and functional studies of the 8-17 DNAzyme

M Cepeda-Plaza, A Peracchi - Organic & Biomolecular Chemistry, 2020 - pubs.rsc.org
DNAzymes (deoxyribozymes) are single-stranded DNA molecules endowed with catalytic
activity, obtained by in vitro selection. In the past 25 years, dozens of DNAzymes have been …

Activity, folding and Z-DNA formation of the 8-17 DNAzyme in the presence of monovalent ions

D Mazumdar, N Nagraj, HK Kim, X Meng… - Journal of the …, 2009 - ACS Publications
The effect of monovalent ions on both the reactivity and global folding of the 8-17 DNAzyme
is investigated, and the results are compared with those of the hammerhead ribozyme …