ABSTRACT RNA interference (RNAi) is an ancient biological mechanism used to defend
against external invasion. It theoretically can silence any disease-related genes in a …

Intracellular delivery is a key step in biological research and has enabled decades of
biomedical discoveries. It is also becoming increasingly important in industrial and medical …

Biomimetic cell membrane-coated nanoparticles (CM-NPs) with superior biochemical
properties have been broadly utilized for various biomedical applications. Currently …

Electroporation (EP) is a commonly used strategy to increase cell permeability for
intracellular cargo delivery or irreversible cell membrane disruption using electric fields. In …

Nuclear transfection of DNA into mammalian cells is challenging yet critical for many
biological and medical studies. Here, by combining cell squeezing and electric-field-driven …

Innate cell function can be artificially engineered and reprogrammed by introducing
biomolecules, such as DNAs, RNAs, plasmid DNAs, proteins, or nanomaterials, into the …

Electroporation is a simple yet powerful technique for breaching the cell membrane barrier.
The applications of electroporation can be generally divided into two categories: the release …

Electroporation (EP) is an effective and widely accepted intracellular delivery method for
fundamental research and medical applications. Existing electroporation methods usually …

Implementation of gene editing technologies such as CRISPR/Cas9 in the manufacture of
novel cell-based therapeutics has the potential to enable highly-targeted, stable, and …

Cancer poses a significant health challenge, with traditional treatments like surgery,
radiotherapy, and chemotherapy often lacking in cell specificity and long‐term curative …