Versatile phospholipid assemblies for functional synthetic cells and artificial tissues

X Wang, H Du, Z Wang, W Mu, X Han - Advanced materials, 2021 - Wiley Online Library
The bottom‐up construction of a synthetic cell from nonliving building blocks capable of
mimicking cellular properties and behaviors helps to understand the particular biophysical …

Growth, replication and division enable evolution of coacervate protocells

AD Slootbeek, MHI van Haren, IBA Smokers… - Chemical …, 2022 - pubs.rsc.org
Living and proliferating cells undergo repeated cycles of growth, replication and division, all
orchestrated by complex molecular networks. How a minimal cell cycle emerged and helped …

Peptide‐based coacervate‐core vesicles with semipermeable membranes

M Abbas, JO Law, SN Grellscheid… - Advanced …, 2022 - Wiley Online Library
Coacervates droplets have long been considered as potential protocells to mimic living
cells. However, these droplets lack a membrane and are prone to coalescence, limiting their …

Transcription and translation in cytomimetic protocells perform most efficiently at distinct macromolecular crowding conditions

MA Vibhute, MH Schaap, RJM Maas… - ACS Synthetic …, 2020 - ACS Publications
The formation of cytomimetic protocells that capture the physicochemical aspects of living
cells is an important goal in bottom-up synthetic biology. Here, we recreated the crowded …

Structure‐Property Relationships Governing Membrane‐Penetrating Behaviour of Complex Coacervates

T Lu, X Hu, MHI van Haren, E Spruijt, WTS Huck - Small, 2023 - Wiley Online Library
Complex coacervates are phase‐separated liquid droplets composed of oppositely charged
multivalent molecules. The unique material properties of the complex coacervate interior …

Effect of osmotic pressure on pore formation in lipid bilayers by the antimicrobial peptide magainin 2

MM Billah, SK Saha, MMO Rashid, F Hossain… - Physical Chemistry …, 2022 - pubs.rsc.org
Osmotic pressure (Π) induces membrane tension in cells and lipid vesicles, which may affect
the activity of antimicrobial peptides (AMPs) by an unknown mechanism. We recently …

Structural stability of liposome-stabilized oil-in-water pickering emulsions and their fate during in vitro digestion

W Liu, J Liu, LJ Salt, MJ Ridout, J Han, PJ Wilde - Food & function, 2019 - pubs.rsc.org
Most current research on food-relevant Pickering emulsions has been conducted using
inorganic or food-compatible organic particles as emulsifiers. A key challenge is maintaining …

Simulations of morphology control of self‐assembled amphiphilic surfactants

Q Zhu, DR Tree - Journal of Polymer Science, 2023 - Wiley Online Library
One of the grand challenges of amphiphilic self‐assembly is the design of ordered structures
whose morphology or shape can be explicitly and dynamically controlled by adjusting the …

High macromolecular crowding in liposomes from microfluidics

LPB Guerzoni, AVC de Goes, M Kalacheva… - Advanced …, 2022 - Wiley Online Library
The intracellular environment is crowded with macromolecules that influence biochemical
equilibria and biomacromolecule diffusion. The incorporation of such crowding in synthetic …

To close or to collapse: the role of charges on membrane stability upon pore formation

RB Lira, FSC Leomil, RJ Melo, KA Riske… - Advanced …, 2021 - Wiley Online Library
Resealing of membrane pores is crucial for cell survival. Membrane surface charge and
medium composition are studied as defining regulators of membrane stability. Pores are …