The twisted intramolecular charge transfer (TICT) mechanism has guided the development
of numerous bright and sensitive fluorophores. This review briefly overviews the history of …

Molecular photoswitches enable dynamic control of processes with high spatiotemporal
precision, using light as external stimulus, and hence are ideal tools for different research …

Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

The last three decades have brought a revolution in fluorescence microscopy. The
development of new microscopes, fluorescent labels and analysis techniques has pushed …

Organic fluorophores aided by current microscopy imaging modalities are essential for
studying biological systems. Recently, red/near-infrared emitting fluorophores have attracted …

In the realm of biological research, the invention of super-resolution microscopy (SRM) has
enabled the visualization of ultrafine sub-cellular structures and their functions in live cells at …

Recent advances in computer vision and machine learning underpin a collection of
algorithms with an impressive ability to decipher the content of images. These deep learning …

Spectrally distinct fluorophores are desired for multiplexed bioimaging. In particular,
monitoring biological processes in living mammals needs fluorophores that operate in the …

Hybrid small-molecule/protein fluorescent probes are powerful tools for visualizing protein
localization and function in living cells. These hybrid probes are constructed by diverse site …

Fluorogenic probes for bioimaging have become essential tools for life science and
medicine, and the key to their development is a precise understanding of the mechanisms …