Cryo-electron microscopy (cryo-EM) of non-crystalline single particles is a biophysical
technique that can be used to determine the structure of biological macromolecules and …

Single particle cryo-electron microscopy (cryo-EM) has matured into a robust method for the
determination of biological macromolecule structures in the past decade, complementing X …

Cryogenic electron microscopy (cryo-EM) enables structure determination of
macromolecular objects and their assemblies. Although the techniques have been …

Sample purity is central to in vitro studies of protein function and regulation, and to the
efficiency and success of structural studies using techniques such as x-ray crystallography …

Transmission electron microscopy (EM) is a versatile technique that can be used to image
biological specimens ranging from intact eukaryotic cells to individual proteins> 150 kDa …

Huntingtin (HTT) is a large (348 kDa) protein that is essential for embryonic development
and is involved in diverse cellular activities such as vesicular transport, endocytosis …

The proteasome is a validated target for anticancer therapy, and proteasome inhibition is
employed in the clinic for the treatment of tumors and hematological malignancies. Here, we …

Differential scanning fluorimetry (DSF) is a technique that reports protein thermal stability via
the selective recognition of unfolded states by fluorogenic dyes. However, DSF applications …

Cryo-electron microscopy (cryo-EM) can now be used to determine high-resolution structural
information on a diverse range of biological specimens. Recent advances have been driven …

Assembly-line polyketide synthases, such as the 6-deoxyerythronolide B synthase (DEBS),
are large enzyme factories prized for their ability to produce specific and complex polyketide …