A genetically encoded, phage‐displayed cyclic‐peptide library

XS Wang, PHC Chen, JT Hampton… - Angewandte …, 2019‏ - Wiley Online Library
Superior to linear peptides in biological activities, cyclic peptides are considered to have
great potential as therapeutic agents. To identify cyclic‐peptide ligands for therapeutic …

MaveDB: an open-source platform to distribute and interpret data from multiplexed assays of variant effect

D Esposito, J Weile, J Shendure, LM Starita… - Genome biology, 2019‏ - Springer
Multiplex assays of variant effect (MAVEs), such as deep mutational scans and massively
parallel reporter assays, test thousands of sequence variants in a single experiment. Despite …

Molecular evolution of peptides by yeast surface display technology

S Linciano, S Pluda, A Bacchin, A Angelini - MedChemComm, 2019‏ - pubs.rsc.org
Genetically encoded peptides possess unique properties, such as a small molecular weight
and ease of synthesis and modification, that make them suitable to a large variety of …

Deep learning-based bioactive therapeutic peptide generation and screening

H Zhang, KM Saravanan, Y Wei, Y Jiao… - Journal of Chemical …, 2023‏ - ACS Publications
Many bioactive peptides demonstrated therapeutic effects over complicated diseases, such
as antiviral, antibacterial, anticancer, etc. It is possible to generate a large number of …

Nonproteinogenic deep mutational scanning of linear and cyclic peptides

JM Rogers, T Passioura, H Suga - … of the National Academy of Sciences, 2018‏ - pnas.org
High-resolution structure–activity analysis of polypeptides requires amino acid structures
that are not present in the universal genetic code. Examination of peptide and protein …

Dual-color bioluminescent sensor proteins for therapeutic drug monitoring of antitumor antibodies

M van Rosmalen, Y Ni, DFM Vervoort, R Arts… - Analytical …, 2018‏ - ACS Publications
Monitoring the levels of therapeutic antibodies in individual patients would allow patient-
specific dose optimization, with the potential for major therapeutic and financial benefits. Our …

Ratiometric bioluminescent sensor proteins based on intramolecular split luciferase complementation

Y Ni, R Arts, M Merkx - ACS sensors, 2018‏ - ACS Publications
Bioluminescent sensor proteins provide attractive tools for applications ranging from in vivo
imaging to point-of-care testing. Here we introduce a new class of ratiometric bioluminescent …

Lasso-grafting of macrocyclic peptide pharmacophores yields multi-functional proteins

E Mihara, S Watanabe, NK Bashiruddin… - Nature …, 2021‏ - nature.com
Protein engineering has great potential for devising multifunctional recombinant proteins to
serve as next-generation protein therapeutics, but it often requires drastic modifications of …

Protein Grafting Techniques: From Peptide Epitopes to Lasso‐Grafted Neobiologics

M Imai, K Colas, H Suga - ChemPlusChem, 2024‏ - Wiley Online Library
Protein engineering techniques have vastly expanded their domain of impact, notably
following the success of antibodies. Likewise, smaller peptide therapeutics have carved an …

A platform to enhance quantitative single molecule localization microscopy

O Golfetto, DL Wakefield, EE Cacao… - Journal of the …, 2018‏ - ACS Publications
Quantitative single molecule localization microscopy (qSMLM) is a powerful approach to
study in situ protein organization. However, uncertainty regarding the photophysical …