Recent advances in single-molecule tracking and imaging techniques

TD Nguyen, YI Chen, LH Chen… - Annual Review of …, 2023 - annualreviews.org
Since the early 1990s, single-molecule detection in solution at room temperature has
enabled direct observation of single biomolecules at work in real time and under …

Single-particle diffusional fingerprinting: A machine-learning framework for quantitative analysis of heterogeneous diffusion

HD Pinholt, SSR Bohr, JF Iversen… - Proceedings of the …, 2021 - National Acad Sciences
Single-particle tracking (SPT) is a key tool for quantitative analysis of dynamic biological
processes and has provided unprecedented insights into a wide range of systems such as …

Classification of diffusion modes in single-particle tracking data: Feature-based versus deep-learning approach

P Kowalek, H Loch-Olszewska, J Szwabiński - Physical Review E, 2019 - APS
Single-particle trajectories measured in microscopy experiments contain important
information about dynamic processes occurring in a range of materials including living cells …

The decade of super-resolution microscopy of the presynapse

G Nosov, M Kahms, J Klingauf - Frontiers in synaptic neuroscience, 2020 - frontiersin.org
The presynaptic compartment of the chemical synapse is a small, yet extremely complex
structure. Considering its size, most methods of optical microscopy are not able to resolve its …

Classification of particle trajectories in living cells: Machine learning versus statistical testing hypothesis for fractional anomalous diffusion

J Janczura, P Kowalek, H Loch-Olszewska… - Physical Review E, 2020 - APS
Single-particle tracking (SPT) has become a popular tool to study the intracellular transport
of molecules in living cells. Inferring the character of their dynamics is important, because it …

Quantitative evaluation of methods to analyze motion changes in single-particle experiments

G Muñoz-Gil, H Bachimanchi, J Pineda… - arxiv preprint arxiv …, 2023 - arxiv.org
The analysis of live-cell single-molecule imaging experiments can reveal valuable
information about the heterogeneity of transport processes and interactions between cell …

Anomalous subdiffusion in living cells: Bridging the gap between experiments and realistic models through collaborative challenges

M Woringer, I Izeddin, C Favard, H Berry - Frontiers in Physics, 2020 - frontiersin.org
The life of a cell is governed by highly dynamical microscopic processes. Two notable
examples are the diffusion of membrane receptors and the kinetics of transcription factors …

Learning physical properties of anomalous random walks using graph neural networks

H Verdier, M Duval, F Laurent, A Cassé… - Journal of Physics A …, 2021 - iopscience.iop.org
Single particle tracking allows probing how biomolecules interact physically with their
natural environments. A fundamental challenge when analysing recorded single particle …

“Waltz” of Cell Membrane-Coated Nanoparticles on Lipid Bilayers: Tracking Single Particle Rotation in Ligand–Receptor Binding

Y Yu, Y Gao, Y Yu - Acs Nano, 2018 - ACS Publications
Understanding the binding of nanoparticles to receptors on biomembranes is critical to the
development and screening of therapeutic materials. A prevailing understanding is that …

Interferometric scattering (iSCAT) microscopy and related techniques

RW Taylor, V Sandoghdar - Label-free super-resolution microscopy, 2019 - Springer
Interferometric scattering (iSCAT) microscopy is a powerful tool for label-free sensitive
detection and imaging of nanoparticles to high spatiotemporal resolution. As it was born out …