[HTML][HTML] CRISPR/Cas9 systems: delivery technologies and biomedical applications
Y Du, Y Liu, J Hu, X Peng, Z Liu - Asian Journal of Pharmaceutical Sciences, 2023 - Elsevier
The emergence of the clustered regularly interspaced short palindromic repeats
(CRISPR)/CRISPR-associated protein 9 (Cas9) genome-editing system has brought about a …
(CRISPR)/CRISPR-associated protein 9 (Cas9) genome-editing system has brought about a …
CRISPR screening in hematology research: from bulk to single-cell level
The CRISPR genome editing technology has revolutionized the way gene function is
studied. Genome editing can be achieved in single genes or for thousands of genes …
studied. Genome editing can be achieved in single genes or for thousands of genes …
Chromatin context-dependent regulation and epigenetic manipulation of prime editing
We set out to exhaustively characterize the impact of the cis-chromatin environment on
prime editing, a precise genome engineering tool. Using a highly sensitive method for …
prime editing, a precise genome engineering tool. Using a highly sensitive method for …
Hyperexcitability and translational phenotypes in a preclinical mouse model of SYNGAP1-related intellectual disability
Disruption of SYNGAP1 directly causes a genetically identifiable neurodevelopmental
disorder (NDD) called SYNGAP1-related intellectual disability (SRID). Without functional …
disorder (NDD) called SYNGAP1-related intellectual disability (SRID). Without functional …
Optimization of Cas12a for multiplexed genome-scale transcriptional activation
Cas12a CRISPR technology, unlike Cas9, allows for facile multiplexing of guide RNAs from
a single transcript, simplifying combinatorial perturbations. While Cas12a has been …
a single transcript, simplifying combinatorial perturbations. While Cas12a has been …
Enhancer regulatory networks globally connect non-coding breast cancer loci to cancer genes
Background Genetic studies have associated thousands of enhancers with breast cancer
(BC). However, the vast majority have not been functionally characterized. Thus, it remains …
(BC). However, the vast majority have not been functionally characterized. Thus, it remains …
Analysis of single-cell CRISPR perturbations indicates that enhancers predominantly act multiplicatively
A single gene may have multiple enhancers, but how they work in concert to regulate
transcription is poorly understood. To analyze enhancer interactions throughout the …
transcription is poorly understood. To analyze enhancer interactions throughout the …
Functional genomics in stem cell models: considerations and applications
K Shevade, S Peddada, K Mader… - Frontiers in Cell and …, 2023 - frontiersin.org
Protocols to differentiate human pluripotent stem cells have advanced in terms of cell type
specificity and tissue-level complexity over the past 2 decades, which has facilitated human …
specificity and tissue-level complexity over the past 2 decades, which has facilitated human …
Characterization and bioinformatic filtering of ambient gRNAs in single-cell CRISPR screens using CLEANSER
Single-cell RNA sequencing CRISPR (perturb-seq) screens enable high-throughput
investigation of the genome, allowing for characterization of thousands of genomic …
investigation of the genome, allowing for characterization of thousands of genomic …
Advances in computational and experimental approaches for deciphering transcriptional regulatory networks: Understanding the roles of cis‐regulatory elements is …
Understanding the influence of cis‐regulatory elements on gene regulation poses numerous
challenges given complexities stemming from variations in transcription factor (TF) binding …
challenges given complexities stemming from variations in transcription factor (TF) binding …