Single-molecule localization microscopy

M Lelek, MT Gyparaki, G Beliu, F Schueder… - Nature reviews …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

DNA origami

S Dey, C Fan, KV Gothelf, J Li, C Lin, L Liu… - Nature Reviews …, 2021 - nature.com
Biological materials are self-assembled with near-atomic precision in living cells, whereas
synthetic 3D structures generally lack such precision and controllability. Recently, DNA …

Super-resolution microscopy demystified

L Schermelleh, A Ferrand, T Huser, C Eggeling… - Nature cell …, 2019 - nature.com
Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that
restricts the optical resolution to roughly 250 nm and was previously thought to be …

Ångström-resolution fluorescence microscopy

SCM Reinhardt, LA Masullo, I Baudrexel, PR Steen… - Nature, 2023 - nature.com
Fluorescence microscopy, with its molecular specificity, is one of the major characterization
methods used in the life sciences to understand complex biological systems. Super …

Ultra‐high sensitivity mass spectrometry quantifies single‐cell proteome changes upon perturbation

AD Brunner, M Thielert, C Vasilopoulou… - Molecular systems …, 2022 - embopress.org
Single‐cell technologies are revolutionizing biology but are today mainly limited to imaging
and deep sequencing. However, proteins are the main drivers of cellular function and in …

The biological applications of DNA nanomaterials: current challenges and future directions

W Ma, Y Zhan, Y Zhang, C Mao, X **e… - Signal transduction and …, 2021 - nature.com
DNA, a genetic material, has been employed in different scientific directions for various
biological applications as driven by DNA nanotechnology in the past decades, including …

Emerging approaches to DNA data storage: challenges and prospects

A Doricchi, CM Platnich, A Gimpel, F Horn, M Earle… - ACS …, 2022 - ACS Publications
With the total amount of worldwide data skyrocketing, the global data storage demand is
predicted to grow to 1.75× 1014 GB by 2025. Traditional storage methods have difficulties …

Super-resolution microscopy for structural cell biology

S Liu, P Hoess, J Ries - Annual review of biophysics, 2022 - annualreviews.org
Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …

Deep learning massively accelerates super-resolution localization microscopy

W Ouyang, A Aristov, M Lelek, X Hao, C Zimmer - Nature biotechnology, 2018 - nature.com
The speed of super-resolution microscopy methods based on single-molecule localization,
for example, PALM and STORM, is limited by the need to record many thousands of frames …

The nucleolus functions as a phase-separated protein quality control compartment

F Frottin, F Schueder, S Tiwary, R Gupta, R Körner… - Science, 2019 - science.org
INTRODUCTION Cells have evolved quality control mechanisms that operate under normal
growth conditions and during stress to maintain protein homeostasis (proteostasis) and …