Structural biologists have traditionally approached cellular complexity in a reductionist
manner in which the cellular molecular components are fractionated and purified before …

Cryo-electron microscopy (cryo-EM) is rapidly becoming an attractive method in the field of
structural biology. With the exploding popularity of cryo-EM, sample preparation must evolve …

Cryo-electron microscopy (cryoEM) is becoming the preferred method for resolving protein
structures. Low signal-to-noise ratio (SNR) in cryoEM images reduces the confidence and …

Abstract SARS-CoV-2 ORF3a is a putative viral ion channel implicated in autophagy
inhibition, inflammasome activation and apoptosis. 3a protein and anti-3a antibodies are …

Cryo-electron microscopy (cryo-EM) has emerged as a powerful structure determination
technique. Its most prolific branch is single particle analysis (SPA), a method being used in a …

We describe a general method that allows structure determination of small proteins by single-
particle cryo-electron microscopy (cryo-EM). The method is based on the availability of a …

Cyclic guanosine monophosphate (GMP)–adenosine monophosphate (AMP) synthase
(cGAS) recognizes cytosolic foreign or damaged DNA to activate the innate immune …

Phosphorylation of proteins is a ubiquitous mechanism of regulating their function,
localization, or activity. Protein kinases, enzymes that use ATP to phosphorylate protein …

The fast development of single-particle cryogenic electron microscopy (cryo-EM) has made it
more feasible to obtain the 3D structure of well-behaved macromolecules with a molecular …

Cryoelectron microscopy (Cryo-EM) has enabled structural determination of proteins larger
than about 50 kDa, including many intractable by any other method, but it has largely failed …