Liver-on-a-chip: Considerations, advances, and beyond

Z Yang, X Liu, EM Cribbin, AM Kim, JJ Li, KT Yong - Biomicrofluidics, 2022 - pubs.aip.org
The liver is the largest internal organ in the human body with largest mass of glandular
tissue. Modeling the liver has been challenging due to its variety of major functions …

Hepatocyte cultures: From collagen gel sandwiches to microfluidic devices with integrated biosensors

JM de Hoyos-Vega, HJ Hong, G Stybayeva… - APL …, 2021 - pubs.aip.org
Hepatocytes are parenchymal cells of the liver responsible for drug detoxification, urea and
bile production, serum protein synthesis, and glucose homeostasis. Hepatocytes are widely …

Microfluidic 3D hepatic cultures integrated with a droplet-based bioanalysis unit

JM de Hoyos-Vega, AM Gonzalez-Suarez… - Biosensors and …, 2024 - Elsevier
A common challenge in microfluidic cell cultures has to do with analysis of cell function
without replacing a significant fraction of the culture volume and disturbing local …

Long-term maintenance of functional primary human hepatocytes in 3D gelatin matrices produced by solution blow spinning

M Klaas, K Möll, K Mäemets-Allas, M Loog… - Scientific Reports, 2021 - nature.com
Solution blow spinning (SBS) has recently emerged as a novel method that can produce
nano-and microfiber structures suitable for tissue engineering. Gelatin is an excellent …

[HTML][HTML] Setup of human liver-chips integrating 3D models, microwells and a standardized microfluidic platform as proof-of-concept study to support drug evaluation

B Cox, P Barton, R Class, H Coxhead… - Biomaterials and …, 2022 - Elsevier
Human 3D liver microtissues/spheroids are powerful in vitro models to study drug-induced
liver injury (DILI) but the small number of cells per spheroid limits the models' usefulness to …

[HTML][HTML] Bioengineering liver microtissues for modeling non-alcoholic fatty liver disease

N Aasadollahei, N Rezaei, R Golroo, T Agarwal… - EXCLI …, 2023 - ncbi.nlm.nih.gov
Non-alcoholic fatty liver disease (NAFLD) has become the world's most common chronic
liver disease. However, due to the lack of reliable in vitro NAFLD models, drug development …

[HTML][HTML] Function of hepatocyte spheroids in bioactive microcapsules is enhanced by endogenous and exogenous hepatocyte growth factor

K Gwon, D Choi, JM de Hoyos-Vega, H Baskaran… - Bioactive Materials, 2023 - Elsevier
The ability to maintain functional hepatocytes has important implications for bioartificial liver
development, cell-based therapies, drug screening, and tissue engineering. Several …

An Ultrathin Coating of Microcapsules Enhances the Function of Encapsulated Hepatocyte Spheroids

D Choi, K Gwon, JM de Hoyos-Vega… - … Applied Materials & …, 2024 - ACS Publications
Maintaining the differentiated phenotype and function of primary hepatocytes in vitro and in
vivo represents a distinct challenge. Our paper describes microcapsules comprised of a …

In vitro miniaturized tuberculosis spheroid model

S Mukundan, P Singh, A Shah, R Kumar, KC O'neill… - Biomedicines, 2021 - mdpi.com
Tuberculosis (TB) is a public health concern that impacts 10 million people around the
world. Current in vitro models are low throughput and/or lack caseation, which impairs drug …

The science and engineering of stem cell‐derived organoids‐examples from hepatic, biliary, and pancreatic tissues

O Ogoke, M Maloy, N Parashurama - Biological Reviews, 2021 - Wiley Online Library
The field of organoid engineering promises to revolutionize medicine with wide‐ranging
applications of scientific, engineering, and clinical interest, including precision and …