Almost all outer membrane proteins (OMPs) in Gram-negative bacteria contain a β-barrel
domain that spans the outer membrane (OM). To reach the OM, OMPs must be translocated …

Envelope biogenesis and homeostasis in gram-negative bacteria are exceptionally intricate
processes that require a multitude of periplasmic chaperones to ensure cellular survival …

Specialized epitope tags are widely used for detecting, manipulating or purifying proteins,
but often their versatility is limited. Here, we introduce the ALFA-tag, a rationally designed …

Measuring precise concentrations of proteins can provide insights into biological processes.
Here we use efficient protein extraction and sample fractionation, as well as state-of-the-art …

The use of alternative translation initiation sites enables production of more than one protein
from a single gene, thereby expanding the cellular proteome. Although several such …

Protein-protein interactions (PPIs) and multi-protein complexes perform central roles in the
cellular systems of all living organisms. In humans, disruptions of the normal patterns of PPIs …

The spatial and temporal coordination of protein transport is an essential cornerstone of the
bacterial adaptation to different environmental conditions. By adjusting the protein …

Cellular proteomes are dynamic and adjusted to permanently changing conditions by ATP-
fueled proteolytic machineries. Among the five AAA+ proteases in Escherichia coli FtsH is …

In Escherichia coli, proteins found in the periplasm or the outer membrane are exported from
the cytoplasm by the general secretory, Sec, system before they acquire stably folded …

To reach their final destinations, outer membrane proteins (OMPs) of gram-negative bacteria
undertake an eventful journey beginning in the cytosol. Multiple molecular machines …